Anti-TNF Receptor I antibody [MR1-2] (ab8160)

Overview

  • Product nameAnti-TNF Receptor I antibody [MR1-2]
    See all TNF Receptor I primary antibodies
  • Description
    Mouse monoclonal [MR1-2] to TNF Receptor I
  • SpecificityThe antibody reacts with the extra-cellular part of the TNF-RI. It also reacts with the soluble receptor. TNF-RI is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha: Induction of cytotoxicity and other functions are mediated largely via TNF-RI. The antibody crossreacts with rhesus and cynomolgus natural TNF-RI.
  • Tested applicationsIP, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Positive control
    • Frozen sections: Human lymphnodes

Properties

Applications

Our Abpromise guarantee covers the use of ab8160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
IHC-Fr
  • Application notesPlease note: this clone is not the most sensitive tool for investigating TNF-RI. For cells with lower TNF-R numbers (see note regarding use of HeLa cells as positive control) we advise using ab2257: clone H398. The specific property of this antibody is its agonistic property, whereas H398 (ab2257) is antagonistic.

    The antibody is an agonistic antibody useful for cell culture experiments and histology on frozen sections.
    Furthermore it can be used for immunoprecipitation.
    The reactivity of the antibody with cellbound TNF-Receptor is minimally inhibited by high concentrations of TNF-alpha.
    Working dilution for frozen sections is 1:10 - 1:20.
  • Target

    • FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
    • Involvement in diseaseFamilial hibernian fever
      Multiple sclerosis 5
    • Sequence similaritiesContains 1 death domain.
      Contains 4 TNFR-Cys repeats.
    • DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
      Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
    • Post-translational
      modifications
      The soluble form is produced from the membrane form by proteolytic processing.
    • Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD120a antibody
      • CD120a antigen antibody
      • FPF antibody
      • MGC19588 antibody
      • p55 antibody
      • p55 R antibody
      • p55-R antibody
      • p55R antibody
      • p60 antibody
      • TBP1 antibody
      • TBPI antibody
      • TNF R antibody
      • TNF R I antibody
      • TNF R1 antibody
      • TNF R55 antibody
      • TNF-R1 antibody
      • TNF-RI antibody
      • TNFAR antibody
      • TNFR 1 antibody
      • TNFR I antibody
      • TNFR-I antibody
      • TNFR1 antibody
      • TNFR55 antibody
      • TNFR60 antibody
      • TNFRI antibody
      • TNFRSF1a antibody
      • TNFRSF1a antibody
      • TNR1A_HUMAN antibody
      • Tumor necrosis factor alpha receptor antibody
      • Tumor necrosis factor binding protein 1 antibody
      • Tumor necrosis factor receptor 1 antibody
      • Tumor necrosis factor receptor superfamily, member 1A antibody
      • Tumor necrosis factor receptor type 1 antibody
      • Tumor necrosis factor receptor type I antibody
      • Tumor necrosis factor-binding protein 1 antibody
      see all

    References for Anti-TNF Receptor I antibody [MR1-2] (ab8160)

    ab8160 has not yet been referenced specifically in any publications.

    Product Wall

    ab8160 has only been tested in IHC with frozen sections, it has not yet been tested with paraffin embedded sections (PES). If you were to try this antibody on PES, we suggest antigen retrieval and a starting dilution of 1:10. If staining is not suffici...

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    No, this mAb has not yet been tested for reactivity against the bovine receptor.

    Our anti- TNF R1 antbodies have not yet been tested on the canine receptor

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"