Anti-TNF Receptor I antibody (ab19139)
- Product nameAnti-TNF Receptor I antibodySee all TNF Receptor I primary antibodies ...
- DescriptionRabbit polyclonal to TNF Receptor I
- Tested applicationsWB, IHC-P, Flow Cyt, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Fruit fly (Drosophila melanogaster), Monkey
Synthetic peptide: GLVPHLGDREKRDSV, corresponding to amino acids 29-43 of Human TNF Receptor I.
- Positive control
- HeLa heat shocked cell lysate.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1mg/ml Borate buffered saline, pH 8.2
- Concentration information loading...
- PurityProtein G purified
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab19139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/1000. Incubate for 1 hour at RT. Antigen retrieval is not essential but may optimise staining.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa.
ICC/IF: Use at an assay dependent dilution (see PMID: 17210691).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
- Involvement in diseaseFamilial hibernian fever
Multiple sclerosis 5
- Sequence similaritiesContains 1 death domain.
Contains 4 TNFR-Cys repeats.
- DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
modificationsThe soluble form is produced from the membrane form by proteolytic processing.
- Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
- Entrez Gene: 282527 Cow
- Entrez Gene: 403634 Dog
- Entrez Gene: 7132 Human
- Entrez Gene: 21937 Mouse
- Entrez Gene: 397020 Pig
- Entrez Gene: 25625 Rat
- Omim: 191190 Human
- SwissProt: O19131 Cow
- SwissProt: P19438 Human
- SwissProt: P25118 Mouse
- SwissProt: P50555 Pig
- SwissProt: P22934 Rat
- Unigene: 279594 Human
- Unigene: 11119 Rat
- CD120a antibody
- CD120a antigen antibody
- FPF antibody
- MGC19588 antibody
- p55 antibody
- p55 R antibody
- p55-R antibody
- p55R antibody
- p60 antibody
- TBP1 antibody
- TBPI antibody
- TNF R antibody
- TNF R I antibody
- TNF R1 antibody
- TNF R55 antibody
- TNF-R1 antibody
- TNF-RI antibody
- TNFAR antibody
- TNFR 1 antibody
- TNFR I antibody
- TNFR-I antibody
- TNFR1 antibody
- TNFR55 antibody
- TNFR60 antibody
- TNFRI antibody
- TNFRSF1a antibody
- TNFRSF1a antibody
- TNR1A_HUMAN antibody
- Tumor necrosis factor alpha receptor antibody
- Tumor necrosis factor binding protein 1 antibody
- Tumor necrosis factor receptor 1 antibody
- Tumor necrosis factor receptor superfamily, member 1A antibody
- Tumor necrosis factor receptor type 1 antibody
- Tumor necrosis factor receptor type I antibody
- Tumor necrosis factor-binding protein 1 antibody
Anti-TNF Receptor I antibody images
Western blot analysis of mouse RAW 264 cell lysate.
Ab19139 staining human normal placenta. Staining is localized to cell membrane and secreted.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Anti-TNF Receptor I antibody (ab19139) at 1/1000 dilution + Human 293T whole cell lysate at 30000 cells
An HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa (possible non-specific binding),48 kDa (possible non-specific binding).
This image is courtesy of an anonymous Abreview
ab19139 staining TNF Receptor I in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/100 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.
References for Anti-TNF Receptor I antibody (ab19139)
This product has been referenced in:
- Schwingshackl A et al. Regulation of interleukin-6 secretion by the two-pore-domain potassium channel Trek-1 in alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol 304:L276-86 (2013). Mouse . Read more (PubMed: 23275623) »
- Kisiswa L et al. TNFa reverse signaling promotes sympathetic axon growth and target innervation. Nat Neurosci 16:865-73 (2013). Read more (PubMed: 23749144) »