Overview

  • Product nameAnti-TNF Receptor I antibodySee all TNF Receptor I primary antibodies ...
  • Description
    Rabbit polyclonal to TNF Receptor I
  • Tested applicationsWB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Fruit fly (Drosophila melanogaster), Monkey
  • Immunogen

    Synthetic peptide:

    GLVPHLGDREKRDSV

    , corresponding to amino acids 29-43 of Human TNF Receptor I.

  • Positive control
    • HeLa heat shocked cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab19139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
Flow Cyt
ICC/IF
  • Application notesFlow Cyt: Use at an assay dependent concentration.
    IHC-P: 1/1000. Incubate for 1 hour at RT. Antigen retrieval is not essential but may optimise staining.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa.
    ICC/IF: Use at an assay dependent dilution (see PMID: 17210691).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.


  • Target

    • FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
    • Involvement in diseaseFamilial hibernian fever
      Multiple sclerosis 5
    • Sequence similaritiesContains 1 death domain.
      Contains 4 TNFR-Cys repeats.
    • DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
      Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
    • Post-translational
      modifications
      The soluble form is produced from the membrane form by proteolytic processing.
    • Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD120a antibody
      • CD120a antigen antibody
      • FPF antibody
      • MGC19588 antibody
      • p55 antibody
      • p55 R antibody
      • p55-R antibody
      • p55R antibody
      • p60 antibody
      • TBP1 antibody
      • TBPI antibody
      • TNF R antibody
      • TNF R I antibody
      • TNF R1 antibody
      • TNF R55 antibody
      • TNF-R1 antibody
      • TNF-RI antibody
      • TNFAR antibody
      • TNFR 1 antibody
      • TNFR I antibody
      • TNFR-I antibody
      • TNFR1 antibody
      • TNFR55 antibody
      • TNFR60 antibody
      • TNFRI antibody
      • TNFRSF1a antibody
      • TNFRSF1a antibody
      • TNR1A_HUMAN antibody
      • Tumor necrosis factor alpha receptor antibody
      • Tumor necrosis factor binding protein 1 antibody
      • Tumor necrosis factor receptor 1 antibody
      • Tumor necrosis factor receptor superfamily, member 1A antibody
      • Tumor necrosis factor receptor type 1 antibody
      • Tumor necrosis factor receptor type I antibody
      • Tumor necrosis factor-binding protein 1 antibody
      see all

    Anti-TNF Receptor I antibody images

    • Western blot analysis of mouse RAW 264 cell lysate.
    • Ab19139 staining human normal placenta. Staining is localized to cell membrane and secreted.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Anti-TNF Receptor I antibody (ab19139) at 1/1000 dilution + Human 293T whole cell lysate at 30000 cells

      Secondary
      An HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution

      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 45 kDa (possible non-specific binding),48 kDa (possible non-specific binding).

      This image is courtesy of an anonymous Abreview

      See Abreview

    • ab19139 staining TNF Receptor I in Human platelet cells by Flow cytometry.
      Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/100 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.

      See Abreview

    References for Anti-TNF Receptor I antibody (ab19139)

    This product has been referenced in:
    • Weiterer S  et al. Tumor necrosis factor alpha induces a serotonin dependent early increase in ciliary beat frequency and epithelial transport velocity in murine tracheae. PLoS One 9:e91705 (2014). IHC-P ; Mouse . Read more (PubMed: 24626175) »
    • Fujita H  et al. Mechanism underlying I?B kinase activation mediated by the linear ubiquitin chain assembly complex. Mol Cell Biol 34:1322-35 (2014). Read more (PubMed: 24469399) »

    See all 20 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
    Sample Mouse Cell (hepatocytes)
    Specification hepatocytes
    Permeabilization Yes - 0.1% Triton X-100
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 19 2014

    Our guarantee (www.abcam.com/abpromise) will cover pig reactivity for the TNFR1 antibody ab19139, but we do not have the data that demonstrated cross-reactivity. A customer several years ago used this antibody to stain sections of pig placenta and repo...

    Read More
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (RAW264.7)
    Specification RAW264.7
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton + 2% BSA in PBS
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
    Username

    Dr. Mahesh Shivananjappa

    Verified customer

    Submitted Apr 26 2013

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Spleen)
    Specification Spleen
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1 Standard
    Permeabilization No
    Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 17 2012

    Vielen Dank für Ihre Anfrage.
    Die meisten unserer Antikörper wurden nur unter denaturierenden/ reduzierenden WB Bedingungen getestet. Ich möchte daher vorschlagen, die Probe 5 min zu kochen und ein reduzierendes Gel zu verwenden.
    Unsere Standa...

    Read More

    Vielen Dank für Ihren Anruf.

    Ich haben den folgenden Artikel gefunden, welcher trotz Trypsinisierung ein gutes Signal bekommt (und das Signal sogar gegen eine nicht-trypsinisierte Probe vergleicht):

    http://www.ncbi.nlm.nih.gov/...

    Read More

    Thank you for contacting us.

    We currently only carry this one anti-TNFR FITC conjugated.

    OurAnti-TNF Receptor I antibody ab19139 has been tested to react with human tissues in a number of applications. While this product is not ...

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    Thanks for your email!

    Please let me know if you need anything further.

    I have sent you an email containing information from FedEx on how you can return the antibody to Abcam. Please package the antibody as it was received with the frozen blue ice pack.

    Please contact me if you have any questions.

    Thank you for your reply.

    A new vial of ab19139 should be with you by 10.30am tomorrow morning and your new order number is ******.

    If there is anything else I can help you with, or if you have any issues with the new vial of antibo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"