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Synthetic peptide corresponding to Mouse TNF Receptor I aa 29-43.
Our Abpromise guarantee covers the use of ab19139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa.|
|IHC-P||1/1000. Antigen retrieval is not essential but may optimise staining.
Incubate for 1 hour at RT.
|Flow Cyt||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17210691|
This image is courtesy of an anonymous AbreviewBlocking Step :5% Milk + 1% BSA for 16 hours at 4°C
ab19139 staining TNF Receptor I in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/100 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.