Overview
- Product nameAnti-TNF Receptor I antibodySee all TNF Receptor I primary antibodies ...
- DescriptionRabbit polyclonal to TNF Receptor I
- Tested applicationsWB, IHC-P, Flow Cyt, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Fruit fly (Drosophila melanogaster), Monkey
- Immunogen
Synthetic peptide: GLVPHLGDREKRDSV, corresponding to amino acids 29-43 of Human TNF Receptor I.
- Positive controlHeLa heat shocked cell lysate.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1mg/ml Borate buffered saline, pH 8.2 -
Concentration information loading... - PurityProtein G purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab19139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| IHC-P | |
| Flow Cyt | |
| ICC/IF |
IHC-P: 1/1000. Incubate for 1 hour at RT. Antigen retrieval is not essential but may optimise staining.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa.
ICC/IF: Use at an assay dependent dilution (see PMID: 17210691).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
- Involvement in diseaseFamilial hibernian fever (FHF) [MIM:142680]: A hereditary periodic fever syndrome characterized by recurrent fever, abdominal pain, localized tender skin lesions and myalgia. Reactive amyloidosis is the main complication and occurs in 25% of cases. Note=The disease is caused by mutations affecting the gene represented in this entry.
Multiple sclerosis 5 (MS5) [MIM:614810]: A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. An intronic mutation affecting alternative splicing and skipping of exon 6 directs increased expression of isoform 4 a transcript encoding a C-terminally truncated protein which is secreted and may function as a TNF antagonist. - Sequence similaritiesContains 1 death domain.
Contains 4 TNFR-Cys repeats. - DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. - Post-translational
modificationsThe soluble form is produced from the membrane form by proteolytic processing. - Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
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Database links
- Entrez Gene: 282527 Cow
- Entrez Gene: 403634 Dog
- Entrez Gene: 7132 Human
- Entrez Gene: 21937 Mouse
- Entrez Gene: 397020 Pig
- Entrez Gene: 25625 Rat
- Omim: 191190 Human
- SwissProt: O19131 Cow
- SwissProt: P19438 Human
- SwissProt: P25118 Mouse
- SwissProt: P50555 Pig
- SwissProt: P22934 Rat
- Unigene: 279594 Human
- Unigene: 11119 Rat
see all
Target information above from: UniProt accession
P19438
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CD120a antibodyCD120a antigen antibodyFPF antibody
- MGC19588 antibodyp55 antibodyp55 R antibodyp55-R antibodyp55R antibodyp60 antibodyTBP1 antibodyTBPI antibodyTNF R antibodyTNF R I antibodyTNF R1 antibodyTNF R55 antibodyTNF-R1 antibodyTNF-RI antibodyTNFAR antibodyTNFR 1 antibodyTNFR I antibodyTNFR-I antibodyTNFR1 antibodyTNFR55 antibodyTNFR60 antibodyTNFRI antibodyTNFRSF1a antibodyTNFRSF1a antibodyTNR1A_HUMAN antibodyTumor necrosis factor alpha receptor antibodyTumor necrosis factor binding protein 1 antibodyTumor necrosis factor receptor 1 antibodyTumor necrosis factor receptor superfamily, member 1A antibodyTumor necrosis factor receptor type 1 antibodyTumor necrosis factor receptor type I antibodyTumor necrosis factor-binding protein 1 antibody
see all
Anti-TNF Receptor I antibody images
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Western blot - TNF Receptor I antibody (ab19139)Western blot analysis of mouse RAW 264 cell lysate. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TNF Receptor I antibody (ab19139)Ab19139 staining human normal placenta. Staining is localized to cell membrane and secreted.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blot - TNF Receptor I antibody (ab19139)Anti-TNF Receptor I antibody (ab19139) at 1/1000 dilution + Human 293T whole cell lysate at 30000 cells
Secondary
An HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa (possible non-specific binding),48 kDa (possible non-specific binding).This image is courtesy of an anonymous Abreview
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Flow Cytometry - TNF Receptor I antibody (ab19139)Image courtesy of an anonymous Abreview.ab19139 staining TNF Receptor I in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/100 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.
References for Anti-TNF Receptor I antibody (ab19139)
This product has been referenced in:
- Schwingshackl A et al. Regulation of interleukin-6 secretion by the two-pore-domain potassium channel Trek-1 in alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol 304:L276-86 (2013). Mouse . Read more (PubMed: 23275623) »
- Bhattacharjee R et al. cAMP prevents TNF-induced apoptosis through inhibiting DISC complex formation in rat hepatocytes. Biochem Biophys Res Commun 423:85-90 (2012). Rat . Read more (PubMed: 22634003) »
