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Anti-TNF Receptor I antibody (ab7365)

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Overview

  • Product nameAnti-TNF Receptor I antibodySee all TNF Receptor I primary antibodies ...
  • Description
    Rabbit polyclonal to TNF Receptor I
  • SpecificityMouse TNF-R I and the soluble receptor. A minor cross-reactivity with mouse TNF alpha is detectable.
  • Tested applicationsWB, IP, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse
  • EpitopeExtra-cellular part of the receptor.

Properties

Applications

Our Abpromise guarantee covers the use of ab7365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
Flow Cyt
  • Application notesFlow Cyt: 1/50.
    IP: 1/50.
    WB: 1/50.
    The antibody is useful for detection of TNF-R I, both the cell bound and soluble form.
    The reactivity of the antibodies with cell-bound or soluble TNF-Receptor is not inhibited by high concentrations of TNF-á.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
    • Involvement in diseaseFamilial hibernian fever (FHF) [MIM:142680]: A hereditary periodic fever syndrome characterized by recurrent fever, abdominal pain, localized tender skin lesions and myalgia. Reactive amyloidosis is the main complication and occurs in 25% of cases. Note=The disease is caused by mutations affecting the gene represented in this entry.
      Multiple sclerosis 5 (MS5) [MIM:614810]: A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. An intronic mutation affecting alternative splicing and skipping of exon 6 directs increased expression of isoform 4 a transcript encoding a C-terminally truncated protein which is secreted and may function as a TNF antagonist.
    • Sequence similaritiesContains 1 death domain.
      Contains 4 TNFR-Cys repeats.
    • DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
      Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
    • Post-translational
      modifications      The soluble form is produced from the membrane form by proteolytic processing.
    • Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.

      • Target information above from: UniProt accession P19438 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CD120a antibodyCD120a antigen antibodyFPF antibody
          MGC19588 antibodyp55 antibodyp55 R antibodyp55-R antibodyp55R antibodyp60 antibodyTBP1 antibodyTBPI antibodyTNF R antibodyTNF R I antibodyTNF R1 antibodyTNF R55 antibodyTNF-R1 antibodyTNF-RI antibodyTNFAR antibodyTNFR 1 antibodyTNFR I antibodyTNFR-I antibodyTNFR1 antibodyTNFR55 antibodyTNFR60 antibodyTNFRI antibodyTNFRSF1a antibodyTNFRSF1a antibodyTNR1A_HUMAN antibodyTumor necrosis factor alpha receptor antibodyTumor necrosis factor binding protein 1 antibodyTumor necrosis factor receptor 1 antibodyTumor necrosis factor receptor superfamily, member 1A antibodyTumor necrosis factor receptor type 1 antibodyTumor necrosis factor receptor type I antibodyTumor necrosis factor-binding protein 1 antibody
        see all

      References for Anti-TNF Receptor I antibody (ab7365)

      ab7365 has not yet been referenced specifically in any publications.

      Publishing research using ab7365 ? Please let us know so that we can cite the reference in this datasheet

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      Displaying 1 - 4 of 4 results for Abreviews and Q&A

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      I actually have the antibody and it works very well but I have found some things about it that are not in your FAQ so I thought I would add information. On mouse C2C12 cells (skeletal muscle myoblasts) it is very much agonistic. It works better than mou...

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      Many thanks for your very valuable, positive feedback and additional information. I have now included this within our FAQs for other fellow researcher to benefit from. Good luck within your research.

      Could you pl. tell me at what dilutions I shoould use these abs( ab7365 and ab 7364) for western blot. What are the molecular wts at which the band will come.

      Thank you for your email. For Western blotting, it is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. The molecular weight of the predicted human TNF-RI is 48 kDa and ...

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      1. Any idea if this antibody (#7365) can be used to trigger downstream signaling by the cognate receptor (mTNFR-I) efficiently enough to mimic its natural ligand (mTNFalpha)? 2. In general, which of the anti-mouseTNFR-I Abs in Abcam's lineup you believ...

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      1. 7365 is not agonistic. The polyclonal is a good receptor agonist. 2. We propose to use the polyclonal also for this purpose although ab7380 is also useful.

      recently purchased antibodies for TNF receptors(#7364 and #7365). What type of a dilution do you recommend for use with immunohistochemistry? Thanks for your help!

      The concentration of antibody to use is dependent on experimental setting and the sensitivity of the detection system used. We suggest trying a range of dilutions, starting from 1:10. As positive control for immunohistochemistry we advise using TNF s...

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