Anti-TNF Receptor I antibody (ab7365)

Overview

  • Product nameAnti-TNF Receptor I antibodySee all TNF Receptor I primary antibodies ...
  • Description
    Rabbit polyclonal to TNF Receptor I
  • SpecificityMouse TNF-R I and the soluble receptor. A minor cross-reactivity with mouse TNF alpha is detectable.
  • Tested applicationsWB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • EpitopeExtra-cellular part of the receptor.

Properties

Applications

Our Abpromise guarantee covers the use of ab7365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
Flow Cyt
  • Application notesFlow Cyt: 1/50.
    IP: 1/50.
    WB: 1/50.
    The antibody is useful for detection of TNF-R I, both the cell bound and soluble form.
    The reactivity of the antibodies with cell-bound or soluble TNF-Receptor is not inhibited by high concentrations of TNF-á.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
    • Involvement in diseaseFamilial hibernian fever
      Multiple sclerosis 5
    • Sequence similaritiesContains 1 death domain.
      Contains 4 TNFR-Cys repeats.
    • DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
      Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
    • Post-translational
      modifications
      The soluble form is produced from the membrane form by proteolytic processing.
    • Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD120a antibody
      • CD120a antigen antibody
      • FPF antibody
      • MGC19588 antibody
      • p55 antibody
      • p55 R antibody
      • p55-R antibody
      • p55R antibody
      • p60 antibody
      • TBP1 antibody
      • TBPI antibody
      • TNF R antibody
      • TNF R I antibody
      • TNF R1 antibody
      • TNF R55 antibody
      • TNF-R1 antibody
      • TNF-RI antibody
      • TNFAR antibody
      • TNFR 1 antibody
      • TNFR I antibody
      • TNFR-I antibody
      • TNFR1 antibody
      • TNFR55 antibody
      • TNFR60 antibody
      • TNFRI antibody
      • TNFRSF1a antibody
      • TNFRSF1a antibody
      • TNR1A_HUMAN antibody
      • Tumor necrosis factor alpha receptor antibody
      • Tumor necrosis factor binding protein 1 antibody
      • Tumor necrosis factor receptor 1 antibody
      • Tumor necrosis factor receptor superfamily, member 1A antibody
      • Tumor necrosis factor receptor type 1 antibody
      • Tumor necrosis factor receptor type I antibody
      • Tumor necrosis factor-binding protein 1 antibody
      see all

    References for Anti-TNF Receptor I antibody (ab7365)

    ab7365 has not yet been referenced specifically in any publications.

    Product Wall

    Many thanks for your very valuable, positive feedback and additional information. I have now included this within our FAQs for other fellow researcher to benefit from. Good luck within your research.

    Thank you for your email. For Western blotting, it is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. The molecular weight of the predicted human TNF-RI is 48 kDa and...

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    1. 7365 is not agonistic. The polyclonal is a good receptor agonist. 2. We propose to use the polyclonal also for this purpose although ab7380 is also useful.

    The concentration of antibody to use is dependent on experimental setting and the sensitivity of the detection system used. We suggest trying a range of dilutions, starting from 1:10. As positive control for immunohistochemistry we advise using TN...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"