Anti-TNF Receptor I antibody (ab7630)

Overview

  • Product nameAnti-TNF Receptor I antibodySee all TNF Receptor I primary antibodies ...
  • Description
    Rabbit polyclonal to TNF Receptor I
  • SpecificityDoes not recognize human sTNFRp75. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the TNFRp55 found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid. This antibody can be used for FACS analysis. Caution should be exhibited as the F(c) domain of the rabbit IgG molecule may interact with cells non-specifically.
  • Tested applicationsDot Blot, ELISA, Flow Cyt, IP, RIA, WBmore details
  • Species reactivity
    Reacts with: Rabbit
  • Immunogen

    Recombinant human sTNFRp55 expressed in Chinese Hamster Cells.

  • EpitopeExtracellular domain of the cell bound human TNFRp55

Properties

Applications

Our Abpromise guarantee covers the use of ab7630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot
ELISA
Flow Cyt
IP
RIA
WB
  • Application notesDot: Use at an assay dependant dilution.
    ELISA: 1/2000 - 1/8000 (against soluble extracellular sTNFRp55 using goat anti-rabbit HRP, ab6721).
    Flow Cyt: Use at an assay dependant dilution.
    IP: 1/400 - 1/800.
    RIA: 1/8000.
    WB: 1/200 - 1/400 using goat anti-rabbit (HRP) ab6721.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
    • Involvement in diseaseFamilial hibernian fever
      Multiple sclerosis 5
    • Sequence similaritiesContains 1 death domain.
      Contains 4 TNFR-Cys repeats.
    • DomainThe domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
      Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
    • Post-translational
      modifications
      The soluble form is produced from the membrane form by proteolytic processing.
    • Cellular localizationCell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
    • Information by UniProt
    • Alternative names
      • CD120a antibody
      • CD120a antigen antibody
      • FPF antibody
      • MGC19588 antibody
      • p55 antibody
      • p55 R antibody
      • p55-R antibody
      • p55R antibody
      • p60 antibody
      • TBP1 antibody
      • TBPI antibody
      • TNF R antibody
      • TNF R I antibody
      • TNF R1 antibody
      • TNF R55 antibody
      • TNF-R1 antibody
      • TNF-RI antibody
      • TNFAR antibody
      • TNFR 1 antibody
      • TNFR I antibody
      • TNFR-I antibody
      • TNFR1 antibody
      • TNFR55 antibody
      • TNFR60 antibody
      • TNFRI antibody
      • TNFRSF1a antibody
      • TNFRSF1a antibody
      • TNR1A_HUMAN antibody
      • Tumor necrosis factor alpha receptor antibody
      • Tumor necrosis factor binding protein 1 antibody
      • Tumor necrosis factor receptor 1 antibody
      • Tumor necrosis factor receptor superfamily, member 1A antibody
      • Tumor necrosis factor receptor type 1 antibody
      • Tumor necrosis factor receptor type I antibody
      • Tumor necrosis factor-binding protein 1 antibody
      see all

    References for Anti-TNF Receptor I antibody (ab7630)

    ab7630 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (TF-1 cells)
    Total protein in input 30 µg
    Specification TF-1 cells
    Immuno-precipitation step Protein A/G
    Username

    Dr. Alison Taylor

    Verified customer

    Submitted Nov 08 2006

    Thank you for your enquiry. We have several antibodies that have been shown to work by immunoprecipitation and western blotting against human receptors 1 and 2. I am assuming that the problem that you are having is due to the fact that you are usin...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"