Overview

  • Product name
    Anti-TNF Receptor I antibody
    See all TNF Receptor I primary antibodies
  • Description
    Rabbit polyclonal to TNF Receptor I
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (GLSTVPDLLL PLVLLELLVG IYPSGVIGLV PHLGDREKRD SVCPQGKYIH) of Human TNF Receptor I (NP_001056).

  • Positive control
    • DU145 cell lysate

Applications

Our Abpromise guarantee covers the use of ab90121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
  • Involvement in disease
    Familial hibernian fever
    Multiple sclerosis 5
  • Sequence similarities
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain
    The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
    Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
  • Post-translational
    modifications
    The soluble form is produced from the membrane form by proteolytic processing.
  • Cellular localization
    Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD120a antibody
    • FPF antibody
    • MGC19588 antibody
    • p55 antibody
    • p55-R antibody
    • p60 antibody
    • TBP1 antibody
    • TBPI antibody
    • TNF R antibody
    • TNF R55 antibody
    • TNF-R1 antibody
    • TNF-RI antibody
    • TNFAR antibody
    • TNFR-I antibody
    • TNFR1 antibody
    • TNFR55 antibody
    • TNFR60 antibody
    • TNFRI antibody
    • TNFRSF1a antibody
    • TNR1A_HUMAN antibody
    • Tumor necrosis factor receptor 1 antibody
    • Tumor necrosis factor receptor superfamily, member 1A antibody
    • Tumor necrosis factor receptor type 1 antibody
    • Tumor necrosis factor receptor type I antibody
    • Tumor necrosis factor-binding protein 1 antibody
    see all

Images

  • Anti-TNF Receptor I antibody (ab90121) at 1 µg/ml (in 5% skim milk / PBS buffer) + DU145 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Gel concentration: 12%
  • ab90121 at 10µg/ml staining TNF Receptor I in human lymphocytes by Immunocytochemistry/ Immunofluorescence. Nuclei were counterstained with DAPI.

References

ab90121 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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