Overview

  • Product nameAnti-TNF Receptor II antibodySee all TNF Receptor II primary antibodies ...
  • Description
    Rabbit polyclonal to TNF Receptor II
  • Tested applicationsWB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (Mouse) (C terminal).

  • Positive control
    • Thymus

Properties

Applications

Our Abpromise guarantee covers the use of ab15563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.
IP Use a concentration of 2 µg/ml.
IHC-P 1/50.

Target

  • FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
  • Sequence similaritiesContains 4 TNFR-Cys repeats.
  • Post-translational
    modifications
    Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
    A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 120b antibody
    • CD120b antibody
    • CD120b antigen antibody
    • Etanercept antibody
    • p75 antibody
    • p75TNFR antibody
    • p80 TNF alpha receptor antibody
    • p80 TNF-alpha receptor antibody
    • TBP-2 antibody
    • TBP2 antibody
    • TBPII antibody
    • TNF R II antibody
    • TNF R2 antibody
    • TNF R75 antibody
    • TNF-R2 antibody
    • TNF-RII antibody
    • TNFBR antibody
    • TNFR 2 antibody
    • TNFR-II antibody
    • TNFR2 antibody
    • TNFR75 antibody
    • TNFR80 antibody
    • TNFRII antibody
    • TNFRSF 1B antibody
    • TNFRSF1B antibody
    • TNR1B antibody
    • TNR1B_HUMAN antibody
    • Tumor necrosis factor beta receptor antibody
    • Tumor necrosis factor binding protein 2 antibody
    • Tumor necrosis factor receptor 2 antibody
    • Tumor necrosis factor receptor superfamily member 1B precursor antibody
    • Tumor necrosis factor receptor type II antibody
    • Tumor necrosis factor-binding protein 2 antibody
    see all

Anti-TNF Receptor II antibody images

  • ab15563 staining TNF Receptor II at 1/50 dilution in thymus by Immunohistochemistry (FFPE-sections).
  • Anti-TNF Receptor II antibody (ab15563) at 1/1000 dilution + whole cell lysate prepared from human platelets (treated with ADP for 30 minutes) at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
    developed using the ECL technique

    Predicted band size : 55 kDa


    Exposure time : 2 minutes

    Image courtesy of Dr Mahesh Shivananjappa by Abreview.

    See Abreview

References for Anti-TNF Receptor II antibody (ab15563)

This product has been referenced in:
  • Kim JE  et al. Status Epilepticus Induces Vasogenic Edema via Tumor Necrosis Factor-a/ Endothelin-1-Mediated Two Different Pathways. PLoS One 8:e74458 (2013). WB, ICC/IF ; Rat . Read more (PubMed: 24040253) »
  • Sakumoto R  et al. Localization of gene and protein expressions of tumor necrosis factor-{alpha} and tumor necrosis factor receptor types I and II in the bovine corpus luteum during the estrous cycle. J Anim Sci 89:3040-7 (2011). Read more (PubMed: 21551345) »

See all 6 Publications for this product

Product Wall

Thank you for contacting us.

For ab15563, the protocol states for antigen retrieval "Boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Wash 3 times in buffer."

For ab1215...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (CD14+)
Loading amount 20 µg
Specification CD14+
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.6% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 06 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Monocytes from Apheresis)
Specification Monocytes from Apheresis
Fixation BD Cytofix/Cytoperm 20 min
Permeabilization Yes - BD Cytofix/Cytoperm 20 min
Gating Strategy Monocytes
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 13 2011

Application Western blot
Sample Human Cell lysate - whole cell (Platelets)
Loading amount 20 µg
Specification Platelets
Treatment ADP for 30 min
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Nov 23 2011

Thank you for your inquiry. Unfortunately, we are not able to provide the blocking peptides for these antibodies. We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion...

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Thank you for contacting us. This antibody has been successfully tested in Dog and Human species in our lab, but has not been tested in Mouse. However, due to the high sequence homology between the protein’s immunogens, it is predicted to react...

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Thank you for your enquiry. I was not able to obtain a range for the sequence of this immunogen, but by looking closely at this antibody's data sheet in immunogen section, it states that the immunogen is a: "Synthetic peptide (the amino acid sequenc...

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Application Immunoprecipitation
Sample Human Cell lysate - whole cell (TF-1 cells)
Total protein in input 30 µg
Specification TF-1 cells
Immuno-precipitation step Protein A/G
Username

Dr. Alison Taylor

Verified customer

Submitted Nov 08 2006

Thank you for your enquiry. The following antibodies fir your specifications (although we don't know if they have been tested on brain samples), please take a look at the online datasheets for additional information. Please contact us again if you have...

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Thank you for your email and I'm sorry to hear that you are experiencing difficulty with these two antibodies. For ab15563, it is suggested on the online datasheet to use thymus as a positive control. Also, TNF receptors are present on nearly all cell ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"