Overview
- Product nameAnti-TNF Receptor II antibodySee all TNF Receptor II primary antibodies ...
- DescriptionRabbit polyclonal to TNF Receptor II
- Tested applicationsWB, IP, IHC-P more details
- Species reactivityReacts with: Dog, Human
Predicted to work with: Mouse, Rat - Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (Mouse) (C terminal).
- Positive controlThymus
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab15563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa. |
| IP | IP: Use a concentration of 2 µg/ml. |
| IHC-P | IHC-P: 1/50. |
Target
- FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
- Sequence similaritiesContains 4 TNFR-Cys repeats.
- Post-translational
modificationsPhosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. - Cellular localizationSecreted and Cell membrane.
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Database links
- Entrez Gene: 7133 Human
- Entrez Gene: 21938 Mouse
- Entrez Gene: 156767 Rat
- Omim: 191191 Human
- SwissProt: P20333 Human
- SwissProt: P25119 Mouse
- SwissProt: Q80WY6 Rat
- Unigene: 256278 Human
- Unigene: 235328 Mouse
- Unigene: 83633 Rat
see all
Target information above from: UniProt accession
P20333
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- CD 120b antibodyCD120b antibodyCD120b antigen antibody
- Etanercept antibodyp75 antibodyp75TNFR antibodyp80 TNF alpha receptor antibodyp80 TNF-alpha receptor antibodyTBP-2 antibodyTBP2 antibodyTBPII antibodyTNF R II antibodyTNF R2 antibodyTNF R75 antibodyTNF-R2 antibodyTNF-RII antibodyTNFBR antibodyTNFR 2 antibodyTNFR-II antibodyTNFR2 antibodyTNFR75 antibodyTNFR80 antibodyTNFRII antibodyTNFRSF 1B antibodyTNFRSF1B antibodyTNR1B antibodyTNR1B_HUMAN antibodyTumor necrosis factor beta receptor antibodyTumor necrosis factor binding protein 2 antibodyTumor necrosis factor receptor 2 antibodyTumor necrosis factor receptor superfamily member 1B precursor antibodyTumor necrosis factor receptor type II antibodyTumor necrosis factor-binding protein 2 antibody
see all
Anti-TNF Receptor II antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TNF Receptor II antibody (ab15563)ab15563 staining TNF Receptor II at 1/50 dilution in thymus by Immunohistochemistry (FFPE-sections). -
Western blot - Anti-TNF Receptor II antibody (ab15563)Anti-TNF Receptor II antibody (ab15563) at 1/1000 dilution + whole cell lysate prepared from human platelets (treated with ADP for 30 minutes) at 20 µg
Secondary
HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
developed using the ECL technique
Predicted band size : 55 kDa
Exposure time : 2 minutesImage courtesy of Dr Mahesh Shivananjappa by Abreview.
References for Anti-TNF Receptor II antibody (ab15563)
This product has been referenced in:
- Sakumoto R et al. Localization of gene and protein expressions of tumor necrosis factor-{alpha} and tumor necrosis factor receptor types I and II in the bovine corpus luteum during the estrous cycle. J Anim Sci 89:3040-7 (2011). Read more (PubMed: 21551345) »
- Jiang B et al. Neuroinflammation in advanced canine glaucoma. Mol Vis 16:2092-108 (2010). IHC-P ; Dog . Read more (PubMed: 21042562) »
