You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-TNF Receptor II antibody (ab7364)

Submit an Abreview Q&A (4)

Overview

  • Product nameAnti-TNF Receptor II antibodySee all TNF Receptor II primary antibodies ...
  • Description
    Rabbit polyclonal to TNF Receptor II
  • SpecificityMouse TNF-R II and the soluble receptor. Minimal crossreactivity is present with mouse TNF alpha.
  • Tested applicationsWB, IP, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse
  • EpitopeExtra-cellular part of the receptor.

Properties

Applications

Our Abpromise guarantee covers the use of ab7364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
Flow Cyt
  • Application notesFlow Cyt: Use at an assay dependent concentration.
    IP: Use at an assay dependent concentration.
    WB: Use at an assay dependent concentration.
    The reactivity of the antibodies with cell-bound or soluble TNF-Receptor is not inhibited by high concentrations of TNF-alpha.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
    • Sequence similaritiesContains 4 TNFR-Cys repeats.
    • Post-translational
      modifications      Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
      A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
    • Cellular localizationSecreted and Cell membrane.

      • Target information above from: UniProt accession P20333 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CD 120b antibodyCD120b antibodyCD120b antigen antibody
          Etanercept antibodyp75 antibodyp75TNFR antibodyp80 TNF alpha receptor antibodyp80 TNF-alpha receptor antibodyTBP-2 antibodyTBP2 antibodyTBPII antibodyTNF R II antibodyTNF R2 antibodyTNF R75 antibodyTNF-R2 antibodyTNF-RII antibodyTNFBR antibodyTNFR 2 antibodyTNFR-II antibodyTNFR2 antibodyTNFR75 antibodyTNFR80 antibodyTNFRII antibodyTNFRSF 1B antibodyTNFRSF1B antibodyTNR1B antibodyTNR1B_HUMAN antibodyTumor necrosis factor beta receptor antibodyTumor necrosis factor binding protein 2 antibodyTumor necrosis factor receptor 2 antibodyTumor necrosis factor receptor superfamily member 1B precursor antibodyTumor necrosis factor receptor type II antibodyTumor necrosis factor-binding protein 2 antibody
        see all

      References for Anti-TNF Receptor II antibody (ab7364)

      ab7364 has not yet been referenced specifically in any publications.

      Publishing research using ab7364 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab7364 Submit a Question for ab7364

      Displaying 1 - 4 of 4 results for Abreviews and Q&A

      10
      Votes: Highest First

      Using the ab7364 TNFR2 antibody for western blot we see a single clear band of the expected (75 kDa) size in spleen lysates of wild type mice. However, we also see an identical band in lysates from TNFR2KO mice. These mice have the neomycin gene inserte...

      Read More

      Thank you for your enquiry and patience, and I'm sorry to hear that you are experiencing difficulty with ab7364. I contacted the originator of this antibody, and it has not been tested before with KO samples. The antibody was raised against highly purif...

      Read More

      Could you pl. tell me at what dilutions I shoould use these abs( ab7365 and ab 7364) for western blot. What are the molecular wts at which the band will come.

      Thank you for your email. For Western blotting, it is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. The molecular weight of the predicted human TNF-RI is 48 kDa and ...

      Read More

      I have tested both TNF-R antibodies ab 7364 and ab 7365 , on mouse tissue with immunoblots and the results are the same 2 bands with both antibodies, one below 50 kDa and one above 100 kDa. That does not fit with the sizes of the TNF R's. any idea/hel...

      Read More

      It is not simple to detect TNF-receptors by western in tissue samples. The receptors are present in low density and we think that you are observing artefacts. The best procedure is to use cells with known high TNF-R density or, as we have done, use rec...

      Read More

      Has this antibody ever been used for immunohistochemistry?

      We do not know of it's use in immunohistochemistry although it works in a number of other applications so it should be OK. If you purchase the antibody and find that it does not work then, in return for your negative data for our datasheets, we can replac...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"