Anti-TNF Receptor II antibody (ab7364)

Overview

  • Product nameAnti-TNF Receptor II antibodySee all TNF Receptor II primary antibodies ...
  • Description
    Rabbit polyclonal to TNF Receptor II
  • SpecificityMouse TNF-R II and the soluble receptor. Minimal crossreactivity is present with mouse TNF alpha.
  • Tested applicationsWB, IP, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse
  • EpitopeExtra-cellular part of the receptor.

Properties

Applications

Our Abpromise guarantee covers the use of ab7364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
Flow Cyt
  • Application notesFlow Cyt: Use at an assay dependent concentration.
    IP: Use at an assay dependent concentration.
    WB: Use at an assay dependent concentration.
    The reactivity of the antibodies with cell-bound or soluble TNF-Receptor is not inhibited by high concentrations of TNF-alpha.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
    • Sequence similaritiesContains 4 TNFR-Cys repeats.
    • Post-translational
      modifications
      Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
      A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
    • Cellular localizationSecreted and Cell membrane.
    • Target information above from: UniProt accession P20333 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CD 120b antibody
      • CD120b antibody
      • CD120b antigen antibody
      • Etanercept antibody
      • p75 antibody
      • p75TNFR antibody
      • p80 TNF alpha receptor antibody
      • p80 TNF-alpha receptor antibody
      • TBP-2 antibody
      • TBP2 antibody
      • TBPII antibody
      • TNF R II antibody
      • TNF R2 antibody
      • TNF R75 antibody
      • TNF-R2 antibody
      • TNF-RII antibody
      • TNFBR antibody
      • TNFR 2 antibody
      • TNFR-II antibody
      • TNFR2 antibody
      • TNFR75 antibody
      • TNFR80 antibody
      • TNFRII antibody
      • TNFRSF 1B antibody
      • TNFRSF1B antibody
      • TNR1B antibody
      • TNR1B_HUMAN antibody
      • Tumor necrosis factor beta receptor antibody
      • Tumor necrosis factor binding protein 2 antibody
      • Tumor necrosis factor receptor 2 antibody
      • Tumor necrosis factor receptor superfamily member 1B precursor antibody
      • Tumor necrosis factor receptor type II antibody
      • Tumor necrosis factor-binding protein 2 antibody
      see all

    References for Anti-TNF Receptor II antibody (ab7364)

    ab7364 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry and patience, and I'm sorry to hear that you are experiencing difficulty with ab7364. I contacted the originator of this antibody, and it has not been tested before with KO samples. The antibody was raised against highly puri...

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    Thank you for your email. For Western blotting, it is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. The molecular weight of the predicted human TNF-RI is 48 kDa and...

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    It is not simple to detect TNF-receptors by western in tissue samples. The receptors are present in low density and we think that you are observing artefacts. The best procedure is to use cells with known high TNF-R density or, as we have done, use ...

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    We do not know of it's use in immunohistochemistry although it works in a number of other applications so it should be OK. If you purchase the antibody and find that it does not work then, in return for your negative data for our datasheets, we can rep...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"