Anti-TNF Receptor II antibody (ab74315)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab74315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human TNF Receptor II peptide (ab74611).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
  • Sequence similaritiesContains 4 TNFR-Cys repeats.
  • Post-translational
    modifications
    Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
    A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 120b antibody
    • CD120b antibody
    • CD120b antigen antibody
    • Etanercept antibody
    • p75 antibody
    • p75TNFR antibody
    • p80 TNF alpha receptor antibody
    • p80 TNF-alpha receptor antibody
    • TBP-2 antibody
    • TBP2 antibody
    • TBPII antibody
    • TNF R II antibody
    • TNF R2 antibody
    • TNF R75 antibody
    • TNF-R2 antibody
    • TNF-RII antibody
    • TNFBR antibody
    • TNFR 2 antibody
    • TNFR-II antibody
    • TNFR2 antibody
    • TNFR75 antibody
    • TNFR80 antibody
    • TNFRII antibody
    • TNFRSF 1B antibody
    • TNFRSF1B antibody
    • TNR1B antibody
    • TNR1B_HUMAN antibody
    • Tumor necrosis factor beta receptor antibody
    • Tumor necrosis factor binding protein 2 antibody
    • Tumor necrosis factor receptor 2 antibody
    • Tumor necrosis factor receptor superfamily member 1B precursor antibody
    • Tumor necrosis factor receptor type II antibody
    • Tumor necrosis factor-binding protein 2 antibody
    see all

Anti-TNF Receptor II antibody images

  • All lanes : Anti-TNF Receptor II antibody (ab74315) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab76828)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab52256)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • All lanes : Anti-TNF Receptor II antibody (ab74315) at 1 µg/ml

    Lane 1 : Heart (Rat) Tissue Lysate
    Lane 2 : Thymus (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa (possible cleavage fragment).

    Exposure time : 8 minutes

References for Anti-TNF Receptor II antibody (ab74315)

ab74315 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Thymus)
Specification Thymus
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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Mr. Antibody Solutions

Verified customer

Submitted May 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"