Overview
- Product nameAnti-TNF Receptor II antibodySee all TNF Receptor II primary antibodies ...
- DescriptionRabbit polyclonal to TNF Receptor II
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human TNF Receptor II.
(Peptide available as ab74611.)
- Positive controlThis antibody gave a positive signal in the following whole cell lysates: Caco 2, HEK293
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab74315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).Can be blocked with TNF Receptor II peptide (ab74611). |
| IHC-P | IHC-P: Use at an assay dependent concentration. |
Target
- FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
- Sequence similaritiesContains 4 TNFR-Cys repeats.
- Post-translational
modificationsPhosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. - Cellular localizationSecreted and Cell membrane.
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Database links
- Entrez Gene: 7133 Human
- Omim: 191191 Human
- SwissProt: P20333 Human
- Unigene: 256278 Human
Target information above from: UniProt accession
P20333
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CD 120b antibodyCD120b antibodyCD120b antigen antibody
- Etanercept antibodyp75 antibodyp75TNFR antibodyp80 TNF alpha receptor antibodyp80 TNF-alpha receptor antibodyTBP-2 antibodyTBP2 antibodyTBPII antibodyTNF R II antibodyTNF R2 antibodyTNF R75 antibodyTNF-R2 antibodyTNF-RII antibodyTNFBR antibodyTNFR 2 antibodyTNFR-II antibodyTNFR2 antibodyTNFR75 antibodyTNFR80 antibodyTNFRII antibodyTNFRSF 1B antibodyTNFRSF1B antibodyTNR1B antibodyTNR1B_HUMAN antibodyTumor necrosis factor beta receptor antibodyTumor necrosis factor binding protein 2 antibodyTumor necrosis factor receptor 2 antibodyTumor necrosis factor receptor superfamily member 1B precursor antibodyTumor necrosis factor receptor type II antibodyTumor necrosis factor-binding protein 2 antibody
see all
Anti-TNF Receptor II antibody images
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Western blot - TNF Receptor II antibody (ab74315)All lanes : Anti-TNF Receptor II antibody (ab74315) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab76828)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab52256)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 48 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes -
Western blot - TNF Receptor II antibody (ab74315)All lanes : Anti-TNF Receptor II antibody (ab74315) at 1 µg/ml
Lane 1 : Heart (Rat) Tissue Lysate
Lane 2 : Thymus (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 48 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible cleavage fragment).
Exposure time : 8 minutes
References for Anti-TNF Receptor II antibody (ab74315)
ab74315 has not yet been referenced specifically in any publications.
