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Overview

  • Product nameTNF Receptor II protein (Active)See all TNF Receptor II proteins and peptides ...
  • Protein descriptionRecombinant fragment corresponding to amino acids 23-253 of human TNF Receptor II fused to the Fc region of human IgG1 (aa 93-330) expressed in modified human 293 cells (Swiss-Prot P20333).
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityThe ND50 of TNF Receptor II-Fc Chimera is typically 20-50 ng/ml as measured by its ability to neutralize TNF-a mediated cytoxicity in murine WEHI 164 cells in the presence of actinomycin D.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS
  • Concentration information loading...
  • Sequence notesTheoretical sequence: LPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQH AKVFCTKTSDTVCDSCED STYTQLWNWVPECLSCGSRC SSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLC A PLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDI CRPHQICNVVAIPGNASMD AVCTSTSPTRSMAPGAVHL PQPVSTRSQHTQPTPEPSTAPSTSFLLPMGPSPPAEGIPK VDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPK DTLMISRTPEVTCVVVDVSHED PEVKFNWYVDGVEVHN AKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNK AL PAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVS LTCLVKGFYPSDIAVEWESNGQPE NNYKTTPPVLDSDG SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLS LSPG K
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83541 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies
    SDS-PAGE
  • Application notesFuncS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    This protein migrates as a band between 55 and 80 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified TNF Receptor II Fc Chimera that has a predicted molecular mass of 51.8 kDa. ab83541 consists of 5-35% carbohydrate by weight.

    This protein separates into a number of isoforms with a pI between 4.5 and 7.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified TNF Receptor II Fc Chimera that has a predicted pI of 7.9.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CD120bp75p75TNFR
        p80 TNF alpha receptorp80 TNF-alpha receptorTBP-2TBPIITNF R2TNF R75TNF-R2TNF-RIITNFR-IITNFR80TNFRIITNFRSF1BTNR1B_HUMANTumor necrosis factor receptor 2Tumor necrosis factor receptor type IITumor necrosis factor-binding protein 2
      see all
  • FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
  • Sequence similaritiesContains 4 TNFR-Cys repeats.
  • Post-translational
    modifications    Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
    A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession P20333 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    TNF Receptor II protein (Active) images

    • Functional Studies - TNF Receptor II protein (Fc Chimera Active) (ab83541)
      Functional Studies - TNF Receptor II protein (Fc Chimera Active) (ab83541)
      Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates that ab83541 exists in multiple isoforms, which differ according to their level of post-translational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins.

      The triangle indicates theoretical pI and MW of the protein.

    References for TNF Receptor II protein (Active) (ab83541)

    ab83541 has not yet been referenced specifically in any publications.

    Publishing research using ab83541 ? Please let us know so that we can cite the reference in this datasheet

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