TNF alpha Monkey ELISA Kit (with color giving dyes) (ab119560)
- Product nameTNF alpha Monkey ELISA Kit (with color giving dyes)See all TNF alpha kits ...
Intra-assay Sample n Mean SD CV% Overall 8%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum
- Assay typeSandwich
- Sensitivity6 U/ml
- Range28.1 U/ml - 1800 U/ml
- Species reactivityReacts with: Monkey
- Product overview
ab119560 TNF alpha Monkey ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of monkey TNF-alpha. ab119560 is for research use only. Not for diagnostic or therapeutic procedures.
Tumor Necrosis Factor is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF alpha also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF alpha. Due to its varied actions throughout the immune system, TNF alpha may play a role in the pathogenesis of many disease states.
TNF alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF alpha within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNF alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Adhesive Films 4 units Assay Buffer Concentrate (20x) 1 x 5ml Biotin-Conjugate anti-monkey TNF alpha polyclonal antibody 1 x 100µl Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml Monkey TNF alpha Standard 2 vials Monoclonal anti-monkey TNF alpha-coated microwell plate 1 unit Red-Dye 1 x 0.4ml Sample Diluent 1 x 12ml Stop Solution 1 x 15ml Streptavidin-HRP 1 x 150µl Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
- APC1 proteinCachectinDIF
- soluble formTnfTNF aTNF alphaTNF macrophage derivedTNF monocyte derivedTNF superfamily member 2 TNF-aTNF-alphaTNFA_HUMANTNFSF2Tumor necrosis factorTumor necrosis factor alphaTumor necrosis factor ligand superfamily member 2
Our Abpromise guarantee covers the use of ab119560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
TNF alpha Monkey ELISA Kit (with color giving dyes) images
Representative standard curve using ab119560.
References for TNF alpha Monkey ELISA Kit (with color giving dyes) (ab119560)
ab119560 has not yet been referenced specifically in any publications.