You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

TNF alpha Monkey ELISA Kit (with color giving dyes) (ab119560)

Submit an Abreview Submit a Question

Overview

  • Product nameTNF alpha Monkey ELISA Kit (with color giving dyes)See all TNF alpha kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich
  • Sensitivity
    6 U/ml
  • Range
    28.1 U/ml - 1800 U/ml
  • Recovery

    90 %

  • Species reactivity
    Reacts with: Monkey
  • Product overview

    ab119560 TNF alpha Monkey ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of monkey TNF-alpha. ab119560 is for research use only. Not for diagnostic or therapeutic procedures.

    Tumor Necrosis Factor is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF alpha also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF alpha. Due to its varied actions throughout the immune system, TNF alpha may play a role in the pathogenesis of many disease states.

    TNF alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF alpha within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNF alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession P01375 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        APC1 proteinCachectinDIF
        soluble formTnfTNF aTNF alphaTNF macrophage derivedTNF monocyte derivedTNF superfamily member 2 TNF-aTNF-alphaTNFA_HUMANTNFSF2Tumor necrosis factorTumor necrosis factor alphaTumor necrosis factor ligand superfamily member 2
      see all

    Applications

    Our Abpromise guarantee covers the use of ab119560 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Sandwich ELISA sELISA

    TNF alpha Monkey ELISA Kit (with color giving dyes) images

    • Sandwich ELISA - TNF alpha Monkey ELISA Kit (with color giving dyes) (ab119560)
      Sandwich ELISA - TNF alpha Monkey ELISA Kit (with color giving dyes) (ab119560)
      Representative standard curve using ab119560.

    Protocols

    References for TNF alpha Monkey ELISA Kit (with color giving dyes) (ab119560)

    ab119560 has not yet been referenced specifically in any publications.

    Publishing research using ab119560 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab119560 Submit a Question for ab119560

    There are currently no Abreviews or Questions for ab119560.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"