You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

TNF alpha Murine ELISA Kit (ab46105)

Submit an Abreview Q&A (1)

Overview

  • Product nameTNF alpha Murine ELISA KitSee all TNF alpha kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 8 553.5pg/ml 11.2 2%
    B 8 124pg/ml 6.4 5.1%
    Inter-assay
    Sample n Mean SD CV%
    A 10 528.7pg/ml 37.3 7%
    B 10 132.2pg/ml 10.8 8.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich
  • Sensitivity
    < 25 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Assay time
    3h 45m
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    INTENDED USE

    The Murine TNF alpha ELISA is to be used for the in-vitro quantitative determination of murine tumor necrosis factor alpha (mTNF alpha) in murine serum, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant murine TNF alpha.

    PRINCIPLE OF THE METHOD

    The murine TNF alpha Kit (mTNF alpha) is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for mTNF alpha has been coated onto the wells of the microtiter strips provided. The antigen and a biotinylated polyclonal antibody specific for mTNF alpha are simultaneously incubated. Revelation step includes Streptavidin-Horse Radish peroxidase and TMB as chromogen.

     

    Recognizes both natural and recombinant Murine TNF alpha

    Range: 31 pg/ml - 1000 pg/ml

    Sensitivity < 25 pg/ml

  • Notes

    Safety Information:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession P01375 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        APC1 proteinCachectinDIF
        soluble formTnfTNF aTNF alphaTNF macrophage derivedTNF monocyte derivedTNF superfamily member 2 TNF-aTNF-alphaTNFA_HUMANTNFSF2Tumor necrosis factorTumor necrosis factor alphaTumor necrosis factor ligand superfamily member 2
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab46105 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      TNF alpha Murine ELISA Kit images

      • ELISA - TNF alpha Murine ELISA Kit (1 x 96 Well Plate) (ab46105)
        ELISA - TNF alpha Murine ELISA Kit (1 x 96 Well Plate) (ab46105)
        Typical mTNF alpha standard curve ranging from 1000 to 31.25 pg/mL

      References for TNF alpha Murine ELISA Kit (ab46105)

      ab46105 has not yet been referenced specifically in any publications.

      Publishing research using ab46105 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab46105 Submit a Question for ab46105

      Displaying 1 - 1 of 1 results for Abreviews and Q&A

      10
      Votes: Highest First

      Will this ELISA detect TNFa in mouse colonic tissue lysate? The lysis buffer contains Tris-HCl, EDTA, Triton-X and protease inhibitor cocktail. The lysate was centrifuged to remove insoluble material.

      Thank you for your inquiry. Yes, this kit can be used on tissue lysates, but your samples may need to be dialyzed in PBS before being assayed. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advi...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"