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Overview

  • Product nameAnti-TNF alpha antibody [28401.111]See all TNF alpha primary antibodies ...
  • Description
    Mouse monoclonal [28401.111] to TNF alpha
  • SpecificityThe antibody does not cross-react with recombinant human TNF-beta, TNF sRI, TNF sRII, nor with recombinant mouse TNF-alpha.
  • Tested applicationsIHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant human tumor necrosis factor alpha

  • Positive controlSpleen

Properties

Applications

Our Abpromise guarantee covers the use of ab33764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
  • Application notesIHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method with 10mM citrate buffer, pH 6.0, before commencing with IHC staining protocol.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
    • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
    • Sequence similaritiesBelongs to the tumor necrosis factor family.
    • Post-translational
      modifications      The soluble form derives from the membrane form by proteolytic processing.
      The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
    • Cellular localizationSecreted and Cell membrane.

      • Target information above from: UniProt accession P01375 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          APC1 antibodyAPC1 protein antibodyCachectin antibody
          DIF antibodyDifferentiation inducing factor antibodyMacrophage cytotoxic factor antibodyMCF antibodyNecrosin antibodyTNF a antibodyTNF alpha antibodyTNF antibodyTNF Macrophage Derived antibodyTNF Monocyte Derived antibodyTNF Superfamily Member 2 antibodyTNF superfamily, member 2 antibodyTNF, macrophage derived antibodyTNF, monocyte derived antibodyTNF-a antibodyTNF-alpha antibodyTNFA antibodyTNFA_HUMAN antibodyTNFSF2 antibodyTumor necrosis factor alpha antibodyTumor necrosis factor antibodyTumor necrosis factor ligand superfamily member 2 antibodyTumor Necrosis Factor Precursor antibodyTumor necrosis factor, soluble form antibodyTumour Necrosis Factor Alpha antibody
        see all

      References for Anti-TNF alpha antibody [28401.111] (ab33764)

      This product has been referenced in:
      • Miura Y  et al. Tumor necrosis factor-related apoptosis-inducing ligand induces neuronal death in a murine model of HIV central nervous system infection. Proc Natl Acad Sci U S A 100:2777-82 (2003). Read more (PubMed: 12601160) »

      See 1 Publication for this product

      Publishing research using ab33764 ? Please let us know so that we can cite the reference in this datasheet

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      no expression in cancer sample or in positive control, PFA/formalin fixed paraffin embedded sections, oral squamous cell carcinoma, spleen - positive control, 10%formalin fixation for 10 hours, Antigen retrieval method - Heat mediated in microwave 10Mm c...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"