Anti-TNF alpha antibody [52B83] (ab1793)

Overview

• Product nameAnti-TNF alpha antibody [52B83]See all TNF alpha primary antibodies ...
• Description
  Mouse monoclonal [52B83] to TNF alpha
• SpecificityThis antibody detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. It reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. It does not react with receptor-bound TNF-alpha. Non-specific background staining is observed in connective tissues.
• Tested applicationsFlow Cyt, IHC-Fr, ICC/IF, ELISA, WB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Guinea pig, Human, Chimpanzee, Zebrafish, Cynomolgus Monkey, Rhesus monkey, Apteronotus leptorhynchus
  
• Immunogen

  Full length native protein (purified) (Human).

• General notesDilution of 20-200 times is useful for IHC on paraffin embedded tissues and frozen sections; flow cytometry and Western blotting. Useful for staining classic paraffin embedded tissues without antigen retrieval.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS with 0.2% BSA, 0.1% sodium azide
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone number52B83
• Myelomaunknown
• IsotypeIgG1
• Light chain typeunknown
• Research Areas
  • Metabolism
  • Types of disease
  • Metabolic disorders

  • Cancer
  • Growth factors
  • TNF

  • Signal Transduction
  • Growth Factors/Hormones
  • TNF

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Immunology
  • Innate Immunity
  • Cytokines
  • TNF Superfamily

Applications

Target

• FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
• Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
• Sequence similaritiesBelongs to the tumor necrosis factor family.
• Post-translational
  modificationsThe soluble form derives from the membrane form by proteolytic processing.
  The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
  O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
• Cellular localizationSecreted and Cell membrane.

Target information above from: UniProt accession P01375 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 100135630 Guinea pig
  • Entrez Gene: 7124 Human
  • Entrez Gene: 21926 Mouse
  • Entrez Gene: 24835 Rat
  • Entrez Gene: 405785 Zebrafish
  • Omim: 191160 Human
  • SwissProt: P51435 Guinea pig
  • SwissProt: P01375 Human

  • SwissProt: P06804 Mouse
  • SwissProt: P16599 Rat
  • Unigene: 241570 Human
  • Unigene: 1293 Mouse
  • Unigene: 2275 Rat

  see all

• Alternative names
  APC1 antibodyAPC1 protein antibodyCachectin antibody

  DIF antibodyDifferentiation inducing factor antibodyMacrophage cytotoxic factor antibodyMCF antibodyNecrosin antibodyTNF a antibodyTNF alpha antibodyTNF antibodyTNF Macrophage Derived antibodyTNF Monocyte Derived antibodyTNF Superfamily Member 2 antibodyTNF superfamily, member 2 antibodyTNF, macrophage derived antibodyTNF, monocyte derived antibodyTNF-a antibodyTNF-alpha antibodyTNFA antibodyTNFA_HUMAN antibodyTNFSF2 antibodyTumor necrosis factor alpha antibodyTumor necrosis factor antibodyTumor necrosis factor ligand superfamily member 2 antibodyTumor Necrosis Factor Precursor antibodyTumor necrosis factor, soluble form antibodyTumour Necrosis Factor Alpha antibody

  see all

Anti-TNF alpha antibody [52B83] images

• 

  Flow Cytometry - TNF alpha antibody [52B83] (ab1793)
  Overlay histogram showing RAW 264.7 cells stained with ab1793 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1793, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
  
  Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TNF alpha antibody [52B83] (ab1793)This image is courtesy of an Abreview submitted by Dr Robert Domitrovic
  ab1793 staining TNF alpha in mouse liver tissue sections, from mice intoxicated with carbon tetrachloride for 7 weeks, by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/50 in 1% BSA) at 4°C for 12 hours. An undiluted HRP-conjugated secondary antibody was used.

  See Abreview

• 

  Immunohistochemistry (Frozen sections) - TNF alpha antibody [52B83] (ab1793)This image is courtesy of an anonymous Abreview
  ab1793 staining TNF alpha in Mouse duodenum and pancreatic cancer associated stroma sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. An Alexa Fluor^®488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were stained by DAPI.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TNF alpha antibody [52B83] (ab1793)This image is courtesy of an anonymous Abreview
  ab1793 staining TNF alpha in Mouse pancreatic neoplasia tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with a MOM kit for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. An undiluted Biotin-conjugated Goat anti-mouse IgG polyclonal (MOM kit) was used as the secondary antibody.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
  ab1793 staining TNFα in RAW 264.7 cells treated with (R)-(-)-rolipram (ab120031), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R)-(-)-rolipram , as described in literature.
  The cells were incubated at 37°C for 24h in media containing different concentrations of ab120031 ((R)-(-)--rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
  ab1793 staining TNFα in RAW 264.7 cells treated with (R,S)-rolipram (ab120029), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R,S)-rolipram, as described in literature.
  The cells were incubated at 37°C for 24h in media containing different concentrations of ab120029 ((R,S)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)

  ab1793 staining TNFα in RAW 264.7 cells treated with (S)-(+)-rolipram (ab120030), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (S)-(+)-rolipram , as described in literature.
  The cells were incubated at 37°C for 24h in media containing different concentrations of ab120030 ((S)-(+)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793(5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.