Overview

  • Product nameAnti-TNF alpha antibody [AS3]See all TNF alpha primary antibodies ...
  • Description
    Mouse monoclonal [AS3] to TNF alpha
  • SpecificityRecognizes human TNF alpha. Does not recognize human TNF beta, mouse TNF alpha or a panel of other human cytokines.
  • Tested applicationsELISA, IPmore details
  • Species reactivity
    Reacts with: Human, Baboon
  • Immunogen

    In vitro cell culture, protein G purified (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab17280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IP
  • Application notes
    ELISA: Use at an assay dependent dilution.
    This antibody has been used as a coating antibody at 5µg/ml in a two site ELISA with biotin conjugated TNF alpha antibody. Typically the ELISA has a working range of 0-10000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 µl. Recovery of human TNF alpha in human serum is typically >85% in the ELISA.
    IP: Use at an assay dependent dilution.
    This antibody has been used to immunoprecipitate >90% TNF alpha in solution using a double-antibody method (Midgley AR & Hepburn MR Use of the double-antibody method to separate antibody bound from free ligand in radioimmunoassay. Methods Enzymol 70:266-74 (1980). PubMed: 7421592).


    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
    • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
    • Sequence similaritiesBelongs to the tumor necrosis factor family.
    • Post-translational
      modifications
      The soluble form derives from the membrane form by proteolytic processing.
      The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • APC1 antibody
      • APC1 protein antibody
      • Cachectin antibody
      • Cachectin antibody
      • DIF antibody
      • DIF antibody
      • Differentiation inducing factor antibody
      • Macrophage cytotoxic factor antibody
      • MCF antibody
      • Necrosin antibody
      • TNF a antibody
      • TNF alpha antibody
      • TNF antibody
      • TNF Macrophage Derived antibody
      • TNF Monocyte Derived antibody
      • TNF Superfamily Member 2 antibody
      • TNF superfamily, member 2 antibody
      • TNF, macrophage derived antibody
      • TNF, monocyte derived antibody
      • TNF-a antibody
      • TNF-alpha antibody
      • TNFA antibody
      • TNFA antibody
      • TNFA_HUMAN antibody
      • TNFSF2 antibody
      • TNFSF2 antibody
      • Tumor necrosis factor alpha antibody
      • Tumor necrosis factor antibody
      • Tumor necrosis factor ligand superfamily member 2 antibody
      • Tumor Necrosis Factor Precursor antibody
      • Tumor Necrosis Factor, Membrane Form antibody
      • Tumor necrosis factor, soluble form antibody
      • Tumour Necrosis Factor Alpha antibody
      see all

    References for Anti-TNF alpha antibody [AS3] (ab17280)

    ab17280 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"