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Recombinant full length protein (Human) from E.coli.
This antibody is is useful in determining the presence of human TNF alpha in various assays. In general, this antibody also detects primate TNF primate in the same formats using similar dilutions. This antibody is also useful for neutralization of human and primate TNF alpha activity in bioassays. It does not neutralize the biological activity of lymphotoxin.
Our Abpromise guarantee covers the use of ab34839 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
It is recommended to incubate the sample with a 1/200 dilution of the antibody for at least 4 hours before being tested.
|ELISA||1/1000 - 1/2000.
This product has been assayed against TNF alpha using HRP conjugated anti-Rabbit IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at RT. This antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody.
|IHC-P||1/200 - 1/400.|
|IHC-Fr||1/200 - 1/400.|
|IP||1/400 - 1/800.
Pre-clearing with a non-specific rabbit IgG is helpful to reduce background.
|WB||1/400 - 1/800. Predicted molecular weight: 17,26 kDa.
Use for supernates or lysates from 2 X 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS.
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
ab34839 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"