Overview

  • Product nameAnti-TNF alpha antibody [B-C7]See all TNF alpha primary antibodies ...
  • Description
    Mouse monoclonal [B-C7] to TNF alpha
  • SpecificityThis antibody is specific for natural and recombinant human TNF alpha. (Recognises TNF alpha bound on its receptor)
  • Tested applicationsBlocking more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab27381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Blocking
  • Application notesBL: Use at an assay dependent dilution.
    Blocks TNF alpha induced cytotoxicity on U937 cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
    • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
    • Sequence similaritiesBelongs to the tumor necrosis factor family.
    • Post-translational
      modifications
      The soluble form derives from the membrane form by proteolytic processing.
      The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
    • Cellular localizationSecreted and Cell membrane.
    • Target information above from: UniProt accession P01375 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • APC1 antibody
      • APC1 protein antibody
      • Cachectin antibody
      • Cachectin antibody
      • DIF antibody
      • DIF antibody
      • Differentiation inducing factor antibody
      • Macrophage cytotoxic factor antibody
      • MCF antibody
      • Necrosin antibody
      • TNF a antibody
      • TNF alpha antibody
      • TNF antibody
      • TNF Macrophage Derived antibody
      • TNF Monocyte Derived antibody
      • TNF Superfamily Member 2 antibody
      • TNF superfamily, member 2 antibody
      • TNF, macrophage derived antibody
      • TNF, monocyte derived antibody
      • TNF-a antibody
      • TNF-alpha antibody
      • TNFA antibody
      • TNFA antibody
      • TNFA_HUMAN antibody
      • TNFSF2 antibody
      • TNFSF2 antibody
      • Tumor necrosis factor alpha antibody
      • Tumor necrosis factor antibody
      • Tumor necrosis factor ligand superfamily member 2 antibody
      • Tumor Necrosis Factor Precursor antibody
      • Tumor Necrosis Factor, Membrane Form antibody
      • Tumor necrosis factor, soluble form antibody
      • Tumour Necrosis Factor Alpha antibody
      see all

    References for Anti-TNF alpha antibody [B-C7] (ab27381)

    This product has been referenced in:
    • Boillot A  et al. Pilot clinical trial of an anti-TNF alpha monoclonal antibody for the treatment of septic shock. Clin Intensive Care 6:52-6 (1995). Read more (PubMed: 10150799) »

    See 1 Publication for this product

    Product Wall

    Thank you for your inquiry.

    The purity was estimated by SDS page higher than 90%.

    I hope this information helps. Please contact us with any other questions.

    The detection antibody is available as ab27381.

    Thank you for your enquiry.

    We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storag...

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    Thank you for contacting us.

    We usually do not have an expiration date for our antibodies, but we have a rule of a thump if the antibody has been stored as instructed on the datasheet:



    Storage at 4C: The antibody expires...

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    We do not know the mechanism of the blocking: Perhaps the antibody blocks the attachment of TNF on this receptor, or blocks the signal transmission after the attachment of the TNF on this receptor. Whatever the mechanism, the result is a blocking ac...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"