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  • Product nameAnti-TNF alpha antibody [CC328] (Biotin)
    See all TNF alpha primary antibodies
  • Description
    Mouse monoclonal [CC328] to TNF alpha (Biotin)
  • ConjugationBiotin
  • SpecificityThis antibody is specific for bovine TNF alpha; a 17.5kDa cytokine.
  • Tested applicationsFlow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Sheep, Cow
  • Immunogen

    Recombinant bovine TNF alpha.

  • General notes

    This biotin conjugate may be used as a detection antibody in ELISA assays for bovine TNF alpha.



Our Abpromise guarantee covers the use of ab35284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.

ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ELISA Use a concentration of 1 - 5 µg/ml.

Membrane permeabilisation is required for ELISA.


  • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • TNF alpha antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

References for Anti-TNF alpha antibody [CC328] (Biotin) (ab35284)

This product has been referenced in:
  • Hay ID  et al. Bioengineering of bacteria to assemble custom-made polyester affinity resins. Appl Environ Microbiol 81:282-91 (2015). Sandwich ELISA . Read more (PubMed: 25344238) »

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