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Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab18696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. b18696 can be used as a capture antibody in sandwich ELISA applications for human TNF-alpha detection in combination with a monoclonal tracer/detection antibody (biotinconjugated). 4 pg/ml of TNF-alpha in serum/plasma or medium can be detected with an assay range of 0 to 2000 pg/ml. Suggested capture coating dose: 1.0-2.0µg/well|
|Neutralising||Use at an assay dependent concentration. ab18696 neutralizes TNF-alpha activity in vitro. Use 2-5 ng of rTNF-alpha per µg of ab18696.|
|IHC-P||Use a concentration of 10 µg/ml. formalin-fixed paraffin-embedded tissues: shows a strong reaction with normal kidney, liver with cirrhosis, and normal lung sample. Positive reaction with lung cancer samples.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"