Anti-TNF alpha antibody [MP6-XT3 ] (ab11564)

Overview

• Product nameAnti-TNF alpha antibody [MP6-XT3 ]See all TNF alpha primary antibodies ...
• Description
  Rat monoclonal [MP6-XT3 ] to TNF alpha
• Specificityab11564 recognises murine TNF alpha. Reactivity with murine TNF Beta has not yet been determined.
• Tested applicationsWB, Flow Cyt, ELISA more details
• Species reactivity
  Reacts with: Mouse, Rat
  
• Immunogen

  Recombinant murine TNF alpha expressed in E. Coli.

• General notesThis product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• PurityProtein G purified
• Purification notesPurified IgG prepared by affinity chromatography from tissue culture supernatant.
• Clonality Monoclonal
• Clone numberMP6-XT3
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Metabolic disorders

  • Cancer
  • Growth factors
  • TNF

  • Signal Transduction
  • Growth Factors/Hormones
  • TNF

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Immunology
  • Innate Immunity
  • Cytokines
  • TNF Superfamily

Applications

Target

• FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
• Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
• Sequence similaritiesBelongs to the tumor necrosis factor family.
• Post-translational
  modificationsThe soluble form derives from the membrane form by proteolytic processing.
  The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
  O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
• Cellular localizationSecreted and Cell membrane.

Target information above from: UniProt accession P01375 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 21926 Mouse
  • Entrez Gene: 24835 Rat
  • SwissProt: P06804 Mouse
  • SwissProt: P16599 Rat
  • Unigene: 1293 Mouse
  • Unigene: 2275 Rat

• Alternative names
  APC1 antibodyAPC1 protein antibodyCachectin antibody

  DIF antibodyDifferentiation inducing factor antibodyMacrophage cytotoxic factor antibodyMCF antibodyNecrosin antibodyTNF a antibodyTNF alpha antibodyTNF antibodyTNF Macrophage Derived antibodyTNF Monocyte Derived antibodyTNF Superfamily Member 2 antibodyTNF superfamily, member 2 antibodyTNF, macrophage derived antibodyTNF, monocyte derived antibodyTNF-a antibodyTNF-alpha antibodyTNFA antibodyTNFA_HUMAN antibodyTNFSF2 antibodyTumor necrosis factor alpha antibodyTumor necrosis factor antibodyTumor necrosis factor ligand superfamily member 2 antibodyTumor Necrosis Factor Precursor antibodyTumor necrosis factor, soluble form antibodyTumour Necrosis Factor Alpha antibody

  see all

Anti-TNF alpha antibody [MP6-XT3 ] images

• 

  Sandwich ELISA - Anti-TNF alpha antibody [MP6-XT3 ] (ab11564)
  Recombinant Mouse TNF alpha detected in a sandwich assay with Rat anti Mouse TNF alpha (ab11564) as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin as the detetction antibody and Streptavidin:HRP as the final layer.