Overview

  • Product nameAnti-TNF alpha antibody [MP6-XT3]See all TNF alpha primary antibodies ...
  • Description
    Rat monoclonal [MP6-XT3] to TNF alpha
  • Specificityab11564 recognises murine TNF alpha. Reactivity with murine TNF Beta has not yet been determined.
  • Tested applicationsWB, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant murine TNF alpha expressed in E. Coli.

  • General notesThis product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. This antibody recognises the 17 kDa soluble form of TNF alpha.
Flow Cyt Use at an assay dependent concentration. Membrane permeabilization is required for this application
ELISA Use a concentration of 5 µg/ml.

Target

  • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • MCF antibody
    • Necrosin antibody
    • TNF a antibody
    • TNF alpha antibody
    • TNF antibody
    • TNF Macrophage Derived antibody
    • TNF Monocyte Derived antibody
    • TNF Superfamily Member 2 antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor Precursor antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    • Tumour Necrosis Factor Alpha antibody
    see all

Anti-TNF alpha antibody [MP6-XT3] images

  • Recombinant Mouse TNF alpha detected in a sandwich assay with Rat anti Mouse TNF alpha (ab11564) as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin as the detetction antibody and Streptavidin:HRP as the final layer.

References for Anti-TNF alpha antibody [MP6-XT3] (ab11564)

This product has been referenced in:
  • Sousa R  et al. Effect of Lactobacillus acidophilus supernatants on body weight and leptin expression in rats. BMC Complement Altern Med 8:5 (2008). Rat . Read more (PubMed: 18284678) »

See 1 Publication for this product

Product Wall

Thank you very much for your inquiry. Reviewing the datasheets for these antibody I am confident that a single ELISPOT should give good results with this combination of antibodies. But unfortunately, these antibodies have not been tested in...

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Thank you for your email. Our source for this antibody tested ab11564 in Western blotting with recombinant TNF alpha. Conditions were standard. To use this antibody in Western blotting, I would recommend referring to our general Western blot protocol w...

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This antibody recognises the 17 kDa soluble form of TNF alpha. TNF alpha forms dimers and trimers, and in addition is found as a 26 kDa integral membrane protein. I am not able to comment on the ability of this antibody to recognise the membrane bound ...

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Thank you for your enquiry and for your patience. All the information that we have regarding these two antibodies is loacted on the online datasheets. To our knowledge, reactivity with TNF beta has still not yet been tested. Mature TNF-beta and TNF-alp...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"