Anti-TNF alpha antibody [NF-2] (ab9813)
- Product nameAnti-TNF alpha antibody [NF-2]See all TNF alpha primary antibodies ...
- DescriptionMouse monoclonal [NF-2] to TNF alpha
- SpecificityRecognizes human TNF alpha. Reacts better with native than SDS-denaturated TNF alpha.
- Tested applicationsIndirect ELISA, Neutralising, WB more details
- Species reactivityReacts with: Human
Recombinant full length protein (Human).
- Positive control
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPBS with 0.1% sodium azide
- Concentration information loading...
- PurityIgG fraction
- Clonality Monoclonal
- Clone numberNF-2
- Light chain typeunknown
- Research Areas
Our Abpromise guarantee covers the use of ab9813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||I-ELISA: 1/1000 - 1/10000.|
|Neutralising||Neut: Use at an assay dependent dilution. At a concentration of 0.5 µg/ml causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.|
|WB||WB: 1/500 - 1/1000.|
- FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
- Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
- Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
- Cellular localizationSecreted and Cell membrane.
- APC1 antibody
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Anti-TNF alpha antibody [NF-2] images
Western blot of human TNF alpha using ab9813 at a concentration of 2 µg/ml.
References for Anti-TNF alpha antibody [NF-2] (ab9813)
This product has been referenced in:
- Renberg B et al. Affibody molecules in protein capture microarrays: evaluation of multidomain ligands and different detection formats. J Proteome Res 6:171-9 (2007). Read more (PubMed: 17203961) »
- Osna N et al. Serum of patients with Guillain-Barre syndrome modulates mitogen-induced cytokine synthesis by normal peripheral blood mononuclear cells. Centr. Eur. J. Immun. 24:82-87 (1999). Read more (PubMed: ) »