Anti-TNF alpha antibody [NF-7] (ab9809)

Overview

• Product nameAnti-TNF alpha antibody [NF-7]See all TNF alpha primary antibodies ...
• Description
  Mouse monoclonal [NF-7] to TNF alpha
• SpecificityRecognizes human TNF alpha.
• Tested applicationsFlow Cyt, ELISA, Indirect ELISA, Neutralising, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length protein (Human).

• General notesThis antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 µg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.

  
  

  This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 µg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS with 0.1% sodium azide
• Concentration information loading...
• PurityIgG fraction
• Primary antibody notes This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 µg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.
• Clonality Monoclonal
• Clone numberNF-7
• MyelomaNS0/1
• IsotypeIgG1
• Light chain typeunknown
• Research Areas
  • Metabolism
  • Types of disease
  • Metabolic disorders

  • Cancer
  • Growth factors
  • TNF

  • Signal Transduction
  • Growth Factors/Hormones
  • TNF

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Immunology
  • Innate Immunity
  • Cytokines
  • TNF Superfamily

Applications

Target

• FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
• Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
• Sequence similaritiesBelongs to the tumor necrosis factor family.
• Post-translational
  modificationsThe soluble form derives from the membrane form by proteolytic processing.
  The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
  O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
• Cellular localizationSecreted and Cell membrane.

Target information above from: UniProt accession P01375 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7124 Human
  • Omim: 191160 Human
  • SwissProt: P01375 Human
  • Unigene: 241570 Human

• Alternative names
  APC1 antibodyAPC1 protein antibodyCachectin antibody

  DIF antibodyDifferentiation inducing factor antibodyMacrophage cytotoxic factor antibodyMCF antibodyNecrosin antibodyTNF a antibodyTNF alpha antibodyTNF antibodyTNF Macrophage Derived antibodyTNF Monocyte Derived antibodyTNF Superfamily Member 2 antibodyTNF superfamily, member 2 antibodyTNF, macrophage derived antibodyTNF, monocyte derived antibodyTNF-a antibodyTNF-alpha antibodyTNFA antibodyTNFA_HUMAN antibodyTNFSF2 antibodyTumor necrosis factor alpha antibodyTumor necrosis factor antibodyTumor necrosis factor ligand superfamily member 2 antibodyTumor Necrosis Factor Precursor antibodyTumor necrosis factor, soluble form antibodyTumour Necrosis Factor Alpha antibody

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Anti-TNF alpha antibody [NF-7] images

• 

  Western blot - TNF alpha antibody [NF-7] (ab9809)
  Western blot of human TNF alpha using ab9809 at a concentration 1 µg/ml.
• 

  Flow Cytometry - Anti-TNF alpha antibody [NF-7] (ab9809)
  Overlay histogram showing THP1 cells stained with ab9809 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9809, 2µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.