Anti-TNF alpha antibody [T1] (ab8162)

Overview

  • Product nameAnti-TNF alpha antibody [T1]See all TNF alpha primary antibodies ...
  • Description
    Mouse monoclonal [T1] to TNF alpha
  • SpecificityThe antibody reacts with free soluble (17kDa) and membrane (26 kDa) human TNF-alpha. The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha.
  • Tested applicationsFlow Cyt, IP, Inhibition Assay, WBmore details
  • Species reactivity
    Reacts with: Human

Properties

Applications

Our Abpromise guarantee covers the use of ab8162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IP
Inhibition Assay
WB
  • Application notesFlow Cyt: Use at an assay dependent concentration.
    IP: Use at an assay dependent concentration.
    Inhib: Use at an assay dependent concentration.
    WB: Used at a dilution of 1/300 on human brain lysate (see Abreview for further information).

    The antibody is useful for inhibition of the biological activity of TNF-alpha. It can be a useful tool to discriminate between the membrane form of TNF expressed on producer cells and the proteolytically cleaved, soluble TNF-alpha bound to its cognate cell membrane receptors (TNF-R I and TNF-R II). For this purpose we recommend to use this antibody in combination with the anti-TNF-alpha antibody ab8163, which recognizes soluble, membrane and receptor bound TNF-alpha.
    The antibody is also useful for immunoprecipitation, immunoassays, flow cytometry and Western blotting.
  • Target

    • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
    • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
    • Sequence similaritiesBelongs to the tumor necrosis factor family.
    • Post-translational
      modifications
      The soluble form derives from the membrane form by proteolytic processing.
      The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • APC1 antibody
      • APC1 protein antibody
      • Cachectin antibody
      • DIF antibody
      • Differentiation inducing factor antibody
      • Macrophage cytotoxic factor antibody
      • MCF antibody
      • Necrosin antibody
      • TNF a antibody
      • TNF alpha antibody
      • TNF antibody
      • TNF Macrophage Derived antibody
      • TNF Monocyte Derived antibody
      • TNF Superfamily Member 2 antibody
      • TNF superfamily, member 2 antibody
      • TNF, macrophage derived antibody
      • TNF, monocyte derived antibody
      • TNF-a antibody
      • TNF-alpha antibody
      • TNFA antibody
      • TNFA_HUMAN antibody
      • TNFSF2 antibody
      • Tumor necrosis factor alpha antibody
      • Tumor necrosis factor antibody
      • Tumor necrosis factor ligand superfamily member 2 antibody
      • Tumor Necrosis Factor Precursor antibody
      • Tumor Necrosis Factor, Membrane Form antibody
      • Tumor necrosis factor, soluble form antibody
      • Tumour Necrosis Factor Alpha antibody
      see all

    References for Anti-TNF alpha antibody [T1] (ab8162)

    ab8162 has not yet been referenced specifically in any publications.

    Product Wall

    The antibodies are not mapped on the epitopes they would recognize. We do not know whether ab16921 is useful, whereas ab8163 has been characterized more specifically. A combination of our antibodies ab8162 and ab8163 can be used for detection of recept...

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    Application Flow Cytometry
    Sample Human Cell (HL cell lines and primary human NK cells)
    Specification HL cell lines and primary human NK cells
    Permeabilization No
    Gating Strategy living cell population
    Username

    Abcam user community

    Verified customer

    Submitted Sep 06 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (Brain)
    Specification Brain
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Jan 31 2006

    Ab8162 is useful for inhibition assays - inhibition of the biological activity of TNF-alpha. However as far as we know this application is not mentioned in any article, yet and we don't have a specific protocol available.

    As far as we are aware, ab8162 has not yet been tested in IHC.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"