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Anti-TNF alpha antibody [TN3-19] (FITC) (ab93588)

Submit an Abreview Q&A (2)Specific References (1)

Overview

  • Product nameAnti-TNF alpha antibody [TN3-19] (FITC)See all TNF alpha primary antibodies ...
  • Description
    Armenian Hamster monoclonal [TN3-19] to TNF alpha (FITC)
  • ConjugationFITC
  • Specificityab93588 reacts with mouse, rat, and rabbit TNF alpha, but not with Human TNF alpha.
  • Tested applicationsFlow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit

    Does not react with

    Human
  • Immunogen

    E. coli expressed recombinant Mouse TNF alpha

  • Positive controlStimulated Mouse splenocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab93588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt
  • Application notesFlow Cyt: Use 0.25µg/test for 105-8 cells in 100µl.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
    • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
    • Sequence similaritiesBelongs to the tumor necrosis factor family.
    • Post-translational
      modifications      The soluble form derives from the membrane form by proteolytic processing.
      The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
    • Cellular localizationSecreted and Cell membrane.

      • Target information above from: UniProt accession P01375 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          APC1 antibodyAPC1 protein antibodyCachectin antibody
          DIF antibodyDifferentiation inducing factor antibodyMacrophage cytotoxic factor antibodyTNF alpha antibodyTNF antibodyTNF superfamily, member 2 antibodyTNF, macrophage derived antibodyTNF, monocyte derived antibodyTNF-a antibodyTNF-alpha antibodyTNFA antibodyTNFA_HUMAN antibodyTNFSF2 antibodyTumor necrosis factor alpha antibodyTumor necrosis factor antibodyTumor necrosis factor ligand superfamily member 2 antibodyTumor necrosis factor, soluble form antibody
        see all

      Anti-TNF alpha antibody [TN3-19] (FITC) images

      • Flow Cytometry - TNF alpha antibody [TN3-19] (FITC) (ab93588)
        Flow Cytometry - TNF alpha antibody [TN3-19] (FITC) (ab93588)
        Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. The cells were surface stained with PE anti-mouse CD4 and intracellularly stained with ab93588.

      References for Anti-TNF alpha antibody [TN3-19] (FITC) (ab93588)

      This product has been referenced in:
      • Sheehan KC  et al. Generation and characterization of hamster monoclonal antibodies that neutralize murine tumor necrosis factors. J Immunol 142:3884-93 (1989). Read more (PubMed: 2469726) »

      See 1 Publication for this product

      Publishing research using ab93588 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab93588 Submit a Question for ab93588

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      Hi,
      On the product sheet of your Anti-TNF alpha antibody [TN3-19] (FITC) (ab93588), it says it is reactive to Rats and one of the applications lists is flow cytometry. Can you please provide me with some literature publications that show this TN3-19 ...

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      Thank you for contacting Abcam.

      All of the flow cytometry data that we have for ab93588 is on the website and datasheet for the product. we know through QC testing that the antibody will react with rat samples, but we do not have data of the ant...

      Read More

      Hello,


      is the following item part of your program and could you submit an offer? For an experiment with rabbit eyes I need a monoclonal Anti-TNF-alpha (rabbit specific).



      Thanks in advance

      Thank you for your inquiry.

      We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com).

      Please enter the name of the protein into t...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"