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Overview

  • Product nameAnti-TNF alpha antibody [V1q(H8)]See all TNF alpha primary antibodies ...
  • Description
    Rat monoclonal [V1q(H8)] to TNF alpha
  • SpecificityAnti-mouse TNF-a reacts with both natural and recombinant TNF-a and shows neutralizing activity.
  • Tested applicationsInhibition Assay, Neutralising, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse

Properties

Applications

Our Abpromise guarantee covers the use of ab8097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Inhibition Assay
Neutralising
Flow Cyt
  • Application notesThe antibody can be used for inhibition of biological activity and FACS analysis.
    Use at an assay dependent concentration.

    Not tested in other applications.

    • Target

    • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
    • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
    • Sequence similaritiesBelongs to the tumor necrosis factor family.
    • Post-translational
      modifications      The soluble form derives from the membrane form by proteolytic processing.
      The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
    • Cellular localizationSecreted and Cell membrane.

      • Target information above from: UniProt accession P01375 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          APC1 antibodyAPC1 protein antibodyCachectin antibody
          DIF antibodyDifferentiation inducing factor antibodyMacrophage cytotoxic factor antibodyMCF antibodyNecrosin antibodyTNF a antibodyTNF a antibodyTNF alpha antibodyTNF antibodyTNF antibodyTNF Macrophage Derived antibodyTNF Monocyte Derived antibodyTNF Superfamily Member 2 antibodyTNF superfamily, member 2 antibodyTNF, macrophage derived antibodyTNF, monocyte derived antibodyTNF-a antibodyTNF-alpha antibodyTNFA antibodyTNFA_HUMAN antibodyTNFSF2 antibodyTumor necrosis factor alpha antibodyTumor necrosis factor antibodyTumor necrosis factor ligand superfamily member 2 antibodyTumor necrosis factor ligand superfamily member 2 antibodyTumor Necrosis Factor Precursor antibodyTumor necrosis factor, soluble form antibodyTumour Necrosis Factor Alpha antibody
        see all

      References for Anti-TNF alpha antibody [V1q(H8)] (ab8097)

      This product has been referenced in:
      • Helk E  et al. TNFa-mediated liver destruction by kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection. PLoS Pathog 9:e1003096 (2013). Neutralising ; Mouse . Read more (PubMed: 23300453) »
      • Goren I  et al. Severely impaired insulin signaling in chronic wounds of diabetic ob/ob mice: a potential role of tumor necrosis factor-alpha. Am J Pathol 168:765-77 (2006). Read more (PubMed: 16507892) »

      See all 3 Publications for this product

      Publishing research using ab8097 ? Please let us know so that we can cite the reference in this datasheet

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