Overview

  • Product nameAnti-TNF alpha antibody
    See all TNF alpha primary antibodies
  • Description
    Rabbit polyclonal to TNF alpha
  • SpecificityThe antibody is directed against mature mouse TNF alpha and is useful in determining its presence in various assays. The antibody will recognize the cell bound precursor of TNF alpha as a 26 kDa protein in immunoblots, particularly in denatured samples. The antibody does not recognize mouse TNF beta (lymphotoxin).
  • Tested applicationsICC/IF, IP, WB, IHC-P, IHC-Fr, ELISA, RIA, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant TNF alpha from mouse, produced in E.coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab34674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IP 1/400 - 1/800.
WB 1/400 - 1/800. Detects a band of approximately 17 kDa (predicted molecular weight: 26 kDa).Can be blocked with Rabbit polyclonal to FILIP1L (ab151331). Detects TNF alpha from supernatants or lysates of 2 x 106 endotoxin stimulated mouse peripheral blood mononuclear cells (PBMC).
IHC-P Use at an assay dependent dilution.
IHC-Fr 1/200.
ELISA 1/1000 - 1/2000. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody.
RIA 1/8000.
Neutralising 1/200. It is recommended to incubate the sample with the dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.

Target

  • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • MCF antibody
    • Necrosin antibody
    • TNF a antibody
    • TNF alpha antibody
    • TNF antibody
    • TNF Macrophage Derived antibody
    • TNF Monocyte Derived antibody
    • TNF Superfamily Member 2 antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor Precursor antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    • Tumour Necrosis Factor Alpha antibody
    see all

Anti-TNF alpha antibody images

  • ab34674 staining TNF alpha in mouse knee joint sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 10 minutes at room temperature followed by incubation with the primary antibody at a 1/200 dilution for 30 minutes. An undiluted HRP-conjugated human anti-rabbit monoclonal was used as secondary antibody.

    See Abreview

  • ab34674 staining TNF alpha in immortalised bone marrow-derived macrophages by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA + 5% goat serum in PBS for 1 hour at 21°C. The primary antibody was diluted 1/200 in the blocking buffer and incubated with the sample for 1 hour at 21°C. The secondary antibody was Alexa Fluor® 568-conjugated Goat anti-Rabbit polyclonal, diluted 1/500.

    See Abreview

References for Anti-TNF alpha antibody (ab34674)

This product has been referenced in:
  • Yu B  et al. Wnt4 signaling prevents skeletal aging and inflammation by inhibiting nuclear factor-?B. Nat Med 20:1009-17 (2014). Read more (PubMed: 25108526) »
  • Zhang Y  et al. Breakdown of immune homeostasis in the testis of mice lacking Tyro3, Axl and Mer receptor tyrosine kinases. Immunol Cell Biol 91:416-26 (2013). Mouse . Read more (PubMed: 23689306) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Rabbit Tissue lysate - whole (aortic tissue)
Specification aortic tissue
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Mouse Tissue sections (Skin)
Specification Skin
Permeabilization Yes - Triton X 100
Fixative perfusion fixation
Username

Abcam user community

Verified customer

Submitted Jul 01 2013

Vielen Dank für Ihre Rückmeldung.

Das Produkt ist unter "TNF alpha peptide (ab151311) " zu finden.

http://www.abcam.com/tnf-alpha-peptide-ab151311.html

Ich hoffe der Link hilft Ihnen weiter. Sollten Sie we...

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Ich freue mich Ihnen mitteilen zu können, dass das TNF alpha peptide (ab151311) jetzt in unseren Produktkatalog aufgenommen wurde.Sie können das Produkt wie gewohnt bei uns Bestellen.

Ich hoffe ich konnte Ihnen helfen. Wenn Sie weite...

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Vielen Dank für Ihre email.

Ich bin mit dem Labor in Kontakt und hoffe das wir Ihnen das zum Anti-TNF alpha antibody (ab34674)gehörende Peptide bald zum Verkauf anbieten können.

Sobald ich die Bestätigung aus dem L...

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Vielen Dank für Ihre Anfrage.

Ich habe mich wegen des TNF alpha Peptides an das Labor gewendet und werde sie informieren sobald ich eine Rückmeldung bekomme ob wir dieses zum Verkauf anbieten können.

Wir legen sehr viel...

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Thank you for your email.

There may be some antibodies where I might have suggested the human tissues or cells. I apologize for this. I have included the positive control information irrespective of species so similar mouse tissues sections ca...

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Thank you for contacting us.

The positive control for ab36392 is IL2 activatedcultured NK cells.

ab9918; human tonsil sections, human cervical squamous cell carcinoma section

ab34674; human artery or colon tissue sections
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Vielen Dank für Ihre Antwortund dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

Es tut mir leid, dass Sie Probleme mit unseren Antikörpern hatten.

Um Ihr Problem zu lösen, m&o...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab34674. To check the status of the order please contact our Customer...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"