Anti-TNF alpha antibody (ab66579)

Overview

• Product nameAnti-TNF alpha antibodySee all TNF alpha primary antibodies ...
• Description
  Rabbit polyclonal to TNF alpha
• Tested applicationsIHC-Fr, WB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Cat, Chimpanzee, Baboon
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TNF alpha.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab66578.)

• Positive controlThis antibody gave a positive signal in the following Lysates: HeLa Whole Cell NIH 3T3 Whole Cell Mouse Liver Tissue HepG2 Whole Cell Rat Liver Tissue

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Metabolic disorders

  • Cancer
  • Growth factors
  • TNF

  • Signal Transduction
  • Growth Factors/Hormones
  • TNF

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Immunology
  • Innate Immunity
  • Cytokines
  • TNF Superfamily

Applications

Target

• FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
• Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
• Sequence similaritiesBelongs to the tumor necrosis factor family.
• Post-translational
  modificationsThe soluble form derives from the membrane form by proteolytic processing.
  The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
  O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
• Cellular localizationSecreted and Cell membrane.

Target information above from: UniProt accession P01375 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7124 Human
  • Entrez Gene: 21926 Mouse
  • Entrez Gene: 24835 Rat
  • Omim: 191160 Human
  • SwissProt: P19101 Cat
  • SwissProt: P01375 Human
  • SwissProt: P06804 Mouse
  • SwissProt: P16599 Rat

  • Unigene: 241570 Human
  • Unigene: 1293 Mouse
  • Unigene: 2275 Rat

  see all

• Alternative names
  APC1 antibodyAPC1 protein antibodyCachectin antibody

  DIF antibodyDifferentiation inducing factor antibodyMacrophage cytotoxic factor antibodyMCF antibodyNecrosin antibodyTNF a antibodyTNF alpha antibodyTNF antibodyTNF macrophage derived antibodyTNF monocyte derived antibodyTNF superfamily member 2 antibodyTNF superfamily, member 2 antibodyTNF, macrophage derived antibodyTNF, monocyte derived antibodyTNF-a antibodyTNF-alpha antibodyTNFA antibodyTNFA_HUMAN antibodyTNFSF2 antibodyTumor necrosis factor alpha antibodyTumor necrosis factor antibodyTumor necrosis factor ligand superfamily member 2 antibodyTumor necrosis factor precursor antibodyTumor necrosis factor, soluble form antibodyTumour necrosis factor alpha antibody

  see all

Anti-TNF alpha antibody images

• 

  Western blot - Anti-TNF alpha antibody (ab66579)
  All lanes : Anti-TNF alpha antibody (ab66579) at 1 µg
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
  Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
  Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 5 : Liver (Rat) Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 25 kDa
  Observed band size : 23 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 48 kDa,68 kDa,75 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TNF alpha antibody (ab66579)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
  ab66579 staining TNF alpha in Human tonsil tissue sections by IHC-P (Formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5 minutes of peroxidase block followed by 10 minutes of protein block at 20°C; antigen retrieval was heat mediated in retrieval solution. Samples were incubated with primary antibody (1/2000 in antibody diluent) for 45 minutes at 20°C. An undiluted HRP-conjugated polymer goat anti-mouse/rabbit IgG polyclonal polymer was used as the secondary antibody.

  See Abreview