SpecificityNo reactivity is detected against human TNF-b (lymphotoxin). This antibody will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin.
Membrane Blocking is recommended with BSA not Milk for this product.
Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli.
Use at an assay dependent concentration. PubMed: 19458984
Use at an assay dependent concentration.
FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
Sequence similaritiesBelongs to the tumor necrosis factor family.
Post-translational modificationsThe soluble form derives from the membrane form by proteolytic processing. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
Tumor necrosis factor ligand superfamily member 2 antibody
Tumor Necrosis Factor, Membrane Form antibody
Tumor necrosis factor, soluble form antibody
Anti-TNF alpha antibody images
Western blot - Anti-TNF alpha antibody (ab6671)
Anti-TNF alpha antibody (ab6671) at 1/1000 dilution + Recombinant TNF-alpha protein
Secondary Goat Anti-Rabbit Dylight® 649 Conjugate at 1/20000 dilution
Additional bands at : 16 kDa (possible cleavage fragment).
Western Blot analysis, labelling TNF-alpha with ab6671 at 1/1000. Blocking was with 1% BSA in TBS-T incubated for 30 minutes at room temperature. The Cleavage Fragment is related to the soluble isoform 77 - 223.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody (ab6671)This image is courtesy of an Abreview submitted by Jing Ma
ab6671 staining TNF alpha in Cynomolgus Monkey dendritic cells/macrophages from inlamed skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH6.0. Samples were incubated with primary antibody (1/100) for 30 minutes at room temperature. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TNF alpha antibody (ab6671)This image is courtesy of an anonymous Abreview
ab6671 staining human artery tissue sections by IHC-P. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 1.5% serum for 10 minutes. The primary antibody was diluted 1/100 and incubated with the sample for 24 hours at 4°C. An HRP-conjugated goat anti-rabbit antibody was used as the secondary.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TNF alpha antibody (ab6671)This image is courtesy of an Abreview submitted by Nicole Schechter
ab6671 staining TNF alpha from human colon by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed prior to blocking in 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 2 hours at 21°C. Alexa fluor® 680 goat polyclonal, diluted 1/5000, was used as the secondary.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody (ab6671)This image is courtesy of an Abreview submitted by Paolo Ronza
Immunohistochemical analysis of formalin-fixed, paraffin embedded fish tissue, staining TNF alpha with ab6671.
Tissue was fixed with Bouin's fixative; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/600 in diluent) for 2 hours. An HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
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