For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab6671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/200 - 1/1000.|
|IHC-P||1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||1/100 - 1/200.|
|WB||1/500 - 1/2000. Can be blocked with Human TNF alpha full length protein (ab140754).
Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli.
|ICC/IF||Use at an assay dependent concentration. PubMed: 19458984|
|IHC-FoFr||Use at an assay dependent concentration.|
ab6671 staining TNF alpha in Cynomolgus Monkey dendritic cells/macrophages from inlamed skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH6.0. Samples were incubated with primary antibody (1/100) for 30 minutes at room temperature. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.
ab6671 staining human artery tissue sections by IHC-P. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 1.5% serum for 10 minutes. The primary antibody was diluted 1/100 and incubated with the sample for 24 hours at 4°C. An HRP-conjugated goat anti-rabbit antibody was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"