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Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab6671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/200 - 1/1000.|
|IHC-P||1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||1/100 - 1/200.|
|WB||1/100 - 1/200. Can be blocked with Human TNF alpha full length protein (ab140754). Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli.|
|ICC/IF||Use at an assay dependent dilution. PubMed: 19458984|
|IHC-FoFr||Use at an assay dependent dilution.|
ab6671 staining TNF alpha in Cynomolgus Monkey dendritic cells/macrophages from inlamed skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH6.0. Samples were incubated with primary antibody (1/100) for 30 minutes at room temperature. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.
ab6671 staining human artery tissue sections by IHC-P. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 1.5% serum for 10 minutes. The primary antibody was diluted 1/100 and incubated with the sample for 24 hours at 4°C. An HRP-conjugated goat anti-rabbit antibody was used as the secondary.
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