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Overview

  • Product nameAnti-TNF alpha antibody
    See all TNF alpha primary antibodies
  • Description
    Rabbit polyclonal to TNF alpha
  • SpecificityNo reactivity is detected against human TNF-b (lymphotoxin). This antibody will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin.
  • Tested applicationsELISA, IHC-P, IHC-Fr, Neutralising, WB, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human, Pig, Fish, Cynomolgus Monkey
    Predicted to work with: Dog, Monkey, Non Human Primates
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
  • General notesEndotoxin content by LAL is <10 pg/ml.

Properties

Applications

Our Abpromise guarantee covers the use of ab6671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/200 - 1/1000.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/100 - 1/200.
Neutralising 1/200.
WB 1/500 - 1/2000. Can be blocked with Human TNF alpha full length protein (ab140754).

Membrane Blocking is recommended with BSA not Milk for this product.

Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. 

ICC/IF Use at an assay dependent concentration. PubMed: 19458984
IHC-FoFr Use at an assay dependent concentration.

Target

  • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • TNF alpha antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

Anti-TNF alpha antibody images

  • Anti-TNF alpha antibody (ab6671) at 1/1000 dilution + Recombinant TNF-alpha protein

    Secondary
    Goat Anti-Rabbit Dylight® 649 Conjugate at 1/20000 dilution

    Additional bands at : 16 kDa (possible cleavage fragment).

    Western Blot analysis, labelling TNF-alpha with ab6671 at 1/1000. Blocking was with 1% BSA in TBS-T incubated for 30 minutes at room temperature. The Cleavage Fragment is related to the soluble isoform 77 - 223. 

  • ab6671 staining TNF alpha in Cynomolgus Monkey dendritic cells/macrophages from inlamed skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH6.0. Samples were incubated with primary antibody (1/100) for 30 minutes at room temperature. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.

    See Abreview

  • ab6671 staining human artery tissue sections by IHC-P.  Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 1.5% serum for 10 minutes.  The primary antibody was diluted 1/100 and incubated with the sample for 24 hours at 4°C.  An HRP-conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab6671 staining TNF alpha from human colon by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed prior to blocking in 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 2 hours at 21°C. Alexa fluor® 680 goat polyclonal, diluted 1/5000, was used as the secondary.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded fish tissue, staining TNF alpha with ab6671.

    Tissue was fixed with Bouin's fixative; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/600 in diluent) for 2 hours. An HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

References for Anti-TNF alpha antibody (ab6671)

This product has been referenced in:
  • Zhang S  et al. A cystic focus filled with soft tissue in femoral head from a haemophilic arthropathy patient: a case report from China. Haemophilia 21:e80-3 (2015). Read more (PubMed: 25333668) »
  • de La Serre CB  et al. Chronic exposure to low dose bacterial lipopolysaccharide inhibits leptin signaling in vagal afferent neurons. Physiol Behav 139:188-94 (2015). WB ; Rat . Read more (PubMed: 25446227) »

See all 32 Publications for this product

Product Wall

Application Western blot
Sample Chicken Tissue lysate - whole (thymus)
Gel Running Conditions Reduced Denaturing (10-20% Tris-glycine)
Loading amount 20 µg
Specification thymus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Jenny Shoots

Verified customer

Submitted Dec 07 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 30 min
Permeabilization No
Specification brain
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 28 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung tissue, macrophages, monocytes)
Antigen retrieval step None
Permeabilization No
Specification Lung tissue, macrophages, monocytes
Blocking step Zytomed Systems HRP Polymer Kit Blocking Reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative Hope-fixation
Username

Abcam user community

Verified customer

Submitted Oct 23 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer 1X
Permeabilization No
Specification kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 14 2015

Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 29 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Sample Rabbit Tissue sections (iliac)
Specification iliac
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 04 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 26 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20 min
Sample Human Tissue sections (testis, colon CA)
Specification testis, colon CA
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 17 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Guinea pig Cell (Lung and Heart)
Specification Lung and Heart
Permeabilization No
Fixative Formalin
Username

Matthew Williams

Verified customer

Submitted Jan 30 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Sample Mouse Tissue sections (Uterus)
Specification Uterus
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted Nov 13 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"