Overview
- Product nameAnti-TNF alpha antibodySee all TNF alpha primary antibodies ...
- DescriptionRabbit polyclonal to TNF alpha
- SpecificityNo reactivity is detected against human TNF-b (lymphotoxin). This antibody will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin.
- Tested applicationsELISA, IHC-P, IHC-Fr, Neutralising, WB, ICC/IF, IHC-FoFr more details
- Species reactivityReacts with: Mouse, Chicken, Guinea pig, Dog, Human, Pig, Fish
Predicted to work with: Monkey, Non Human Primates - Immunogen
Recombinant full length protein (Human).
- General notesEndotoxin content by LAL is <10 pg/ml.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2 -
Concentration information loading... - PurityIgG fraction
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab6671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/200 - 1/1000. |
| IHC-P | IHC-P: 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| IHC-Fr | IHC-Fr: 1/100 - 1/200. |
| Neutralising | Neut: 1/200. |
| WB | WB: 1/100 - 1/200. Can be blocked with TNF alpha protein (ab140754). Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. PubMed: 19458984 |
| IHC-FoFr | IHC-FoFr: Use at an assay dependent dilution. |
Target
- FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
- Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
- Sequence similaritiesBelongs to the tumor necrosis factor family.
- Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. - Cellular localizationSecreted and Cell membrane.
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Database links
- Entrez Gene: 403922 Dog
- Entrez Gene: 100135630 Guinea pig
- Entrez Gene: 7124 Human
- Entrez Gene: 21926 Mouse
- Entrez Gene: 397086 Pig
- Omim: 191160 Human
- SwissProt: P51742 Dog
- SwissProt: P51435 Guinea pig
- SwissProt: P01375 Human
- SwissProt: P06804 Mouse
- SwissProt: P23563 Pig
- Unigene: 241570 Human
- Unigene: 1293 Mouse
see all
Target information above from: UniProt accession
P01375
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- APC1 antibodyAPC1 protein antibodyCachectin antibody
- DIF antibodyDifferentiation inducing factor antibodyMacrophage cytotoxic factor antibodyMCF antibodyNecrosin antibodyTNF a antibodyTNF alpha antibodyTNF antibodyTNF Macrophage Derived antibodyTNF Monocyte Derived antibodyTNF Superfamily Member 2 antibodyTNF superfamily, member 2 antibodyTNF, macrophage derived antibodyTNF, monocyte derived antibodyTNF-a antibodyTNF-alpha antibodyTNFA antibodyTNFA_HUMAN antibodyTNFSF2 antibodyTumor necrosis factor alpha antibodyTumor necrosis factor antibodyTumor necrosis factor ligand superfamily member 2 antibodyTumor Necrosis Factor Precursor antibodyTumor necrosis factor, soluble form antibodyTumour Necrosis Factor Alpha antibody
see all
Anti-TNF alpha antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TNF alpha antibody (ab6671)This image is courtesy of an anonymous Abreviewab6671 staining human artery tissue sections by IHC-P. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 1.5% serum for 10 minutes. The primary antibody was diluted 1/100 and incubated with the sample for 24 hours at 4°C. An HRP-conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TNF alpha antibody (ab6671)This image is courtesy of an Abreview submitted by Nicole Schechterab6671 staining TNF alpha from human colon by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed prior to blocking in 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 2 hours at 21°C. Alexa fluor® 680 goat polyclonal, diluted 1/5000, was used as the secondary. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody (ab6671)This image is courtesy of an Abreview submitted by Paolo RonzaImmunohistochemical analysis of formalin-fixed, paraffin embedded fish tissue, staining TNF alpha with ab6671.
Tissue was fixed with Bouin's fixative; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/600 in diluent) for 2 hours. An HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
References for Anti-TNF alpha antibody (ab6671)
This product has been referenced in:
- Puig KL et al. Amyloid precursor protein and proinflammatory changes are regulated in brain and adipose tissue in a murine model of high fat diet-induced obesity. PLoS One 7:e30378 (2012). WB ; Mouse . Read more (PubMed: 22276186) »
- Shi JQ et al. Anti-TNF-a reduces amyloid plaques and tau phosphorylation and induces CD11c-positive dendritic-like cell in the APP/PS1 transgenic mouse brains. Brain Res 1368:239-47 (2011). WB ; Mouse . Read more (PubMed: 20971085) »
