SpecificityThe antibody reacts with human natural and recombinant TNF-a as assessed by ELISA. The antibody inhibits the biological activity of human natural and recombinant TNF-a as determined with L929 and WEHI cells in a cytotoxicity assay.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesThe antibody is useful for neutralization of human and monkey TNF-a in bio-assays. Furthermore the antibody is useful for staining of TNF-a in Western blots and flow cytometry. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialysed against culture medium to remove the sodium azide added. Dilutions have to be made according to the amount of TNF-a to be inactivated. For western blots the product should be diluted 20 times.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
Sequence similaritiesBelongs to the tumor necrosis factor family.
Post-translational modificationsThe soluble form derives from the membrane form by proteolytic processing. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.