Anti-TNF alpha antibody (ab9348)
- Product nameAnti-TNF alpha antibodySee all TNF alpha primary antibodies ...
- DescriptionMouse monoclonal to TNF alpha
- Tested applicationsELISA, IHC-P more details
- Species reactivityReacts with: Human
Recombinant human TNF-alpha (Tumor Necrosis Factor alpha), catalogue number ab9642.
- Positive control
- IHC-P: FFPE human tonsil tissue sections.
- General notesMonoclonal antibodies were produced in BALB/c x C57BL/6 F1 mice
- FormLyophilised:Reconstitute with 500µl of sterile water.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPhosphate buffered saline
- Concentration information loading...
- PurityIgG fraction
- Purification notesIgG1/K antibody was purified from ammonium sulfate precipitation followed by ion exchange.
- Clonality Monoclonal
- Light chain typekappa
- Research Areas
Our Abpromise guarantee covers the use of ab9348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
A 1:10,000 dilution of a 1mg/ml antibody solution will give satisfactory results in an ELISA.
- FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
- Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
- Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
- Cellular localizationSecreted and Cell membrane.
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
- DIF antibody
- Differentiation inducing factor antibody
- Macrophage cytotoxic factor antibody
- MCF antibody
- Necrosin antibody
- TNF a antibody
- TNF alpha antibody
- TNF antibody
- TNF Macrophage Derived antibody
- TNF Monocyte Derived antibody
- TNF Superfamily Member 2 antibody
- TNF superfamily, member 2 antibody
- TNF, macrophage derived antibody
- TNF, monocyte derived antibody
- TNF-a antibody
- TNF-alpha antibody
- TNFA antibody
- TNFA_HUMAN antibody
- TNFSF2 antibody
- Tumor necrosis factor alpha antibody
- Tumor necrosis factor antibody
- Tumor necrosis factor ligand superfamily member 2 antibody
- Tumor Necrosis Factor Precursor antibody
- Tumor Necrosis Factor, Membrane Form antibody
- Tumor necrosis factor, soluble form antibody
- Tumour Necrosis Factor Alpha antibody
Anti-TNF alpha antibody images
IHC image of TNF alpha staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9348, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-TNF alpha antibody (ab9348)
ab9348 has not yet been referenced specifically in any publications.