Overview

  • Product nameAnti-TNF alpha antibody
    See all TNF alpha primary antibodies
  • Description
    Rabbit polyclonal to TNF alpha
  • Tested applicationsWB, ELISA, Neutralising, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Highly pure (>98%) recombinant rTNF-alpha (rat Tumor Necrosis Factor-alpha).

  • Positive control
    • IF/ICC: PC12 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab9755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Neutralising
ICC/IF
  • Application notesNeutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rTNF-alpha (2.0 ng/ml), a concentration of 0.145 - 0.165 µg/ml of this antibody is required

    ELISA:To detect rTNF-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rTNF-alpha.

    Western Blot:To detect rTNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rTNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
    • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
    • Sequence similaritiesBelongs to the tumor necrosis factor family.
    • Post-translational
      modifications
      The soluble form derives from the membrane form by proteolytic processing.
      The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • APC1 antibody
      • APC1 protein antibody
      • Cachectin antibody
      • DIF antibody
      • Differentiation inducing factor antibody
      • Macrophage cytotoxic factor antibody
      • MCF antibody
      • Necrosin antibody
      • TNF a antibody
      • TNF alpha antibody
      • TNF antibody
      • TNF Macrophage Derived antibody
      • TNF Monocyte Derived antibody
      • TNF Superfamily Member 2 antibody
      • TNF superfamily, member 2 antibody
      • TNF, macrophage derived antibody
      • TNF, monocyte derived antibody
      • TNF-a antibody
      • TNF-alpha antibody
      • TNFA antibody
      • TNFA_HUMAN antibody
      • TNFSF2 antibody
      • Tumor necrosis factor alpha antibody
      • Tumor necrosis factor antibody
      • Tumor necrosis factor ligand superfamily member 2 antibody
      • Tumor Necrosis Factor Precursor antibody
      • Tumor Necrosis Factor, Membrane Form antibody
      • Tumor necrosis factor, soluble form antibody
      • Tumour Necrosis Factor Alpha antibody
      see all

    Anti-TNF alpha antibody images

    • ICC/IF image of ab9755 stained PC12 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9755, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab9755, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-TNF alpha antibody (ab9755)

    ab9755 has not yet been referenced specifically in any publications.

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    Wie am Telefon besprochen, habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 1234567 und solltespätestens Übermorgenbei Ihnen ankommen.
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    Thank you for your enquiry. I have read the details that you have submitted on behalf of your customer and I have a few comments. According to the EXPASY database TNF alpha is mainly secreted by macrophages. As I understand the heart does have ...

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    Thank you for your enquiry. According to the “Swiss Prot” reference for rat the predicted molecular weight is 28KDa so it is likely that the band that you are observing at 25KDa is the target. Have you used the recombinant form of the target as a p...

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    All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). According to the datasheet, this antibody has not been tested for IHC application.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"