Overview
Product nameTNF alpha protein (Active)See all TNF alpha proteins and peptides ...
Protein descriptionRecombinant full length soluble Human TNF alpha (amino acids 77-233) with His tag produced in the endosperm tissue of barley grain (Hordeum vulgare); 174 amino acids with tag, Predicted MWt 19.6 kDa (UniProt P01375).
Expression hostBarley
Properties
Purity> 95
% by SDS-PAGE
Purification notesGreater than 98% by SDS-PAGE gel analysis.
Sterile filtered through a 0.2 µm filter.
Endotoxin level is less than 0.005ng per µg of product (0.05EU/µg) as measured by turbidimetric kinetic assay.
ab89572 carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction.
Biological activityBioactivity: The ED50 is determined by the Cytotoxicity Assay for Human TNF alpha for dose dependent cytolytic effects of serial dilutions of hTNF alpha on the fibrosarcoma L-929 cells. The ED50 for ab89572 was determined to be 40 ng/ml corresponding to 2.5x106 U/mg.
FormLyophilised:Reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA).
Storage instructionsThe lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferPreservative: None
Constituents: PBS, pH 7.2
Concentration information loading...
Additional notesReconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab89572
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
|
| Functional Studies |
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Application notesFuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution. The recombinant protein migrates as two bands with an apparent molecular mass of 21 and 23 kDa in SDS-PAGE.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
APC1APC1 proteinCachectin
DIFDifferentiation inducing factorMacrophage cytotoxic factorTNFTNF alphaTNF superfamily, member 2TNF, macrophage derivedTNF, monocyte derivedTNF-aTNF-alphaTNFATNFA_HUMANTNFSF2Tumor necrosis factorTumor necrosis factor alphaTumor necrosis factor ligand superfamily member 2Tumor necrosis factor, soluble form
see all
FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
Sequence similaritiesBelongs to the tumor necrosis factor family.
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
Cellular localizationSecreted and Cell membrane.
References for TNF alpha protein (Active) (ab89572)
ab89572
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
