Products:Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
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Will your mouse anti-A20 react with mouse A20 in Western blots? |
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ANSWER: |
Thank you for your enquiry. Our two A20 antibodies raised in mice have been tested for Western blotting on human samples. Human A20 is 89% homologous with the sequence for mouse A20. So there is a good chance these antibodies will recognize mouse A20 but we cannot guarantee this. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-TNFAIP3 antibody [59A426] (ab13597) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 15 kDa,34 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Overlay histogram showing HepG2 cells stained with ab13597 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13597, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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