![Western blot - TNFAIP3 antibody [59A426] (ab13597) image](#TNFAIP3-Primary-antibodies-ab13597-4.jpg)
![Flow Cytometry - TNFAIP3 antibody [59A426] (ab13597) image](#TNFAIP3-Primary-antibodies-ab13597-5.jpg)
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Products:Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
Overview
Product name
Anti-TNFAIP3 antibody [59A426]
See all TNFAIP3 products (7) ...
Description
Mouse monoclonal [59A426] to TNFAIP3
Tested applications
IHC-P, Flow Cyt, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fusion protein of full length TNFAIP3 (Human).
Epitope
The epitope has been mapped to the C-terminal portion of A20, amino acids 440-790.
Positive control
Whole cell lysate from Daudi cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
59A426
Isotype
IgG1
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. (PubMed: 19637364)
Flow Cyt: Use 1-2µg for 106 cells.
WB: Use a concentration of 3 - 5 µg/ml.Detects a band of approximately 70 kDa.
Target
Function
Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
Sequence similarities
Belongs to the peptidase C64 family.
Contains 7 A20-type zinc fingers.
Contains 1 OTU domain.
Domain
The A20-type zinc fingers mediate the ubiquitin ligase activity.
The OTU domain mediates the deubiquitinase activity.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionP21580
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A20 antibody
- MGC104522 antibody
- MGC138687 antibody
see all
Database links
- Entrez Gene: 7128 Human
- Omim: 191163 Human
- SwissProt: P21580 Human
- Unigene: 211600 Human
Anti-TNFAIP3 antibody [59A426] images:
Western blot - TNFAIP3 antibody [59A426] (ab13597)
![Western blot - TNFAIP3 antibody [59A426] (ab13597)](/ps/datasheet/images/13/ab13597/TNFAIP3-Primary-antibodies-ab13597-4.jpg)
All lanes : Anti-TNFAIP3 antibody [59A426] (ab13597) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 15 kDa,34 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Flow Cytometry - TNFAIP3 antibody [59A426] (ab13597)
![Flow Cytometry - TNFAIP3 antibody [59A426] (ab13597)](/ps/datasheet/images/13/ab13597/TNFAIP3-Primary-antibodies-ab13597-5.jpg)
Overlay histogram showing HepG2 cells stained with ab13597 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13597, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HepG2 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-TNFAIP3 antibody [59A426] (ab13597)
This product has been referenced in:
- Imbeault Met al. Acquisition of host-derived CD40L by HIV-1 in vivo and its functional consequences in the B-cell compartment. J Virol 85:2189-200 (2011). WB; Human.Read more (PubMed: 21177803) »
- Karube Ket al. Identification of FOXO3 and PRDM1 as tumor-suppressor gene candidates in NK-cell neoplasms by genomic and functional analyses. Blood 118:3195-204 (2011).Read more (PubMed: 21690554) »
See all 8 publications for this product
Publishing research using ab13597? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"