- Datasheet
- Specific References (20)
- Protocols
Overview
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Product nameAnti-TNFAIP3 antibody [59A426]
See all TNFAIP3 primary antibodies -
DescriptionMouse monoclonal [59A426] to TNFAIP3
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Host speciesMouse
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Tested applicationsSuitable for: IHC-P, Flow Cyt, WB, ICC/IF, IPmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fusion protein of full length TNFAIP3 (Human).
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EpitopeThe epitope has been mapped to the C-terminal portion of A20, amino acids 440-790.
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Positive control
- Whole cell lysate from Daudi cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone number59A426
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab13597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. PubMed: 19637364 | |
| Flow Cyt | Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| WB | Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 70 kDa. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IP | Use a concentration of 1 - 2 µg/ml. |
Target
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FunctionUbiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
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Sequence similaritiesBelongs to the peptidase C64 family.
Contains 7 A20-type zinc fingers.
Contains 1 OTU domain. -
DomainThe A20-type zinc fingers mediate the ubiquitin ligase activity.
The OTU domain mediates the deubiquitinase activity. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 7128 Human
- Entrez Gene: 21929 Mouse
- Entrez Gene: 683206 Rat
- Omim: 191163 Human
- SwissProt: P21580 Human
- SwissProt: Q60769 Mouse
- Unigene: 211600 Human
- Unigene: 116683 Mouse
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Alternative names
- A20 antibody
- AISBL antibody
- MGC104522 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [59A426] (ab13597)](http://a.abcam.com/ps/products/13/ab13597/Images/ab13597-206521-ab13597ihcpplacenta.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [59A426] (ab13597)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue labelling TNFAIP3 with ab13597 at 5µg/ml. Staining was enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
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![Western blot - Anti-TNFAIP3 [59A426] antibody (ab13597)](http://a.abcam.com/ps/products/13/ab13597/Images/ab13597-206522-ab13597wb.jpg)
Western blot - Anti-TNFAIP3 [59A426] antibody (ab13597)Anti-TNFAIP3 antibody [59A426] (ab13597) at 4 µg/ml + Jurkat cell lysate -
![Western blot - Anti-TNFAIP3 antibody [59A426] (ab13597)](http://a.abcam.com/ps/products/13/ab13597/Images/TNFAIP3-Primary-antibodies-ab13597-4.jpg)
Western blot - Anti-TNFAIP3 antibody [59A426] (ab13597)All lanes : Anti-TNFAIP3 antibody [59A426] (ab13597) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 90 kDa (why is the actual band size different from the predicted?)
Additional bands at: 15 kDa, 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
![Flow Cytometry - Anti-TNFAIP3 antibody [59A426] (ab13597)](http://a.abcam.com/ps/products/13/ab13597/Images/TNFAIP3-Primary-antibodies-ab13597-5.jpg)
Flow Cytometry - Anti-TNFAIP3 antibody [59A426] (ab13597)Overlay histogram showing HepG2 cells stained with ab13597 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13597, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HepG2 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Protocols
References
This product has been referenced in:
- Meng Z et al. A20 Ameliorates Intracerebral Hemorrhage-Induced Inflammatory Injury by Regulating TRAF6 Polyubiquitination. J Immunol 198:820-831 (2017). Read more (PubMed: 27986908) »
- Xu H et al. Elevated serum A20 is associated with severity of chronic hepatitis B and A20 inhibits NF-?B-mediated inflammatory response. Oncotarget 8:38914-38926 (2017). Read more (PubMed: 28473659) »
Customer reviews and Q&As
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