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Products:Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
Overview
Product name
Anti-TNFAIP3 antibody
See all TNFAIP3 products (7) ...
Description
Chicken polyclonal to TNFAIP3
Tested applications
IHC-Fr, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: LRSSQRRDVP RTTQSTSRPK CARASCKNILA CRSEELCMEC QHPNQRMGPGA HRGEPAPEDP PKQRCRAPAC DHFGNAKCNGY CNECFQFKQMYG, corresponding to amino acids 696-790 of Human TNFAIP3.
LRSSQRRDVP RTTQSTSRPK CARASCKNIL A CRSEEL CMEC QHPNQRMGPG A HRGEPAPEDP PKQRCRAPAC D HFGNAKCNGY CNECFQFKQM YG
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
-
Western blot - TNFAIP3 antibody (ab14223)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 18006655)
WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa (predicted molecular weight: 90 kDa).
Target
Function
Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
Sequence similarities
Belongs to the peptidase C64 family.
Contains 7 A20-type zinc fingers.
Contains 1 OTU domain.
Domain
The A20-type zinc fingers mediate the ubiquitin ligase activity.
The OTU domain mediates the deubiquitinase activity.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionP21580
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A20 antibody
- MGC104522 antibody
- MGC138687 antibody
see all
Database links
- Entrez Gene: 7128 Human
- Entrez Gene: 21929 Mouse
- Omim: 191163 Human
- SwissProt: P21580 Human
- SwissProt: Q60769 Mouse
- Unigene: 211600 Human
- Unigene: 116683 Mouse
Anti-TNFAIP3 antibody images:
Western blot - TNFAIP3 antibody (ab14223)
Predicted band size : 90 kDa
E coli derived fusion protein as antigen. ab14223 dilution 1/2000 goat anti HRP dilution 1/1000.
References for Anti-TNFAIP3 antibody (ab14223)
This product has been referenced in:
- Wolfrum Set al. The protective effect of A20 on atherosclerosis in apolipoprotein E-deficient mice is associated with reduced expression of NF-kappaB target genes. Proc Natl Acad Sci U S A 104:18601-6 (2007). IHC-Fr; Mouse.Read more (PubMed: 18006655) »
See 1 publication for this product
Publishing research using ab14223? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"