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Products:Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
Overview
Product name
Anti-TNFAIP3 antibody
See all TNFAIP3 products (7) ...
Description
Rabbit polyclonal to TNFAIP3
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human TNFAIP3.
(Peptide available as ab45365.)
Positive control
This antibody gave a positive signal in Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - TNFAIP3 antibody (ab45366)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
IHC-P: Use at a dilution of 1/250.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
Sequence similarities
Belongs to the peptidase C64 family.
Contains 7 A20-type zinc fingers.
Contains 1 OTU domain.
Domain
The A20-type zinc fingers mediate the ubiquitin ligase activity.
The OTU domain mediates the deubiquitinase activity.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionP21580
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A20 antibody
- MGC104522 antibody
- MGC138687 antibody
see all
Database links
- Entrez Gene: 7128 Human
- Omim: 191163 Human
- SwissProt: P21580 Human
- Unigene: 211600 Human
Anti-TNFAIP3 antibody images:
Western blot - TNFAIP3 antibody (ab45366)
Anti-TNFAIP3 antibody (ab45366) at 1 µg/ml + Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab3951) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 89 kDa
Observed band size : 89 kDa
Additional bands at : 50 kDa,75 kDa. We are unsure as to the identity of these extra bands.
References for Anti-TNFAIP3 antibody (ab45366)
This product has been referenced in:
- Malinverni Cet al. Cleavage by MALT1 induces cytosolic release of A20. Biochem Biophys Res Commun 400:543-7 (2010). WB; Human.Read more (PubMed: 20804738) »
See 1 publication for this product
Publishing research using ab45366? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"