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Western blot - TNFAIP3 antibody (ab45366)
(enlarge)
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Product Name
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TNFAIP3 antibody
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See all TNFAIP3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TNFAIP3
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human TNFAIP3. (Peptide available as ab45365.)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a dilution of 1/250.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate
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Cellular localization
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Cytoplasm. Nucleus.
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Research areas
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Cancer >> Growth factors >> TNF
Signal Transduction >> Growth Factors/Hormones >> TNF
Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
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Relevance
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TNFAIP3 interacts with NAF1 and inhibits TNF induced NF kappa B dependent gene expression by interfering with a RIP or TRAF2 mediated transactivation signal. It inhibits programmed cell death, has a role in the function of the lymphoid system, and may contribute to the in vivo effects of TNF.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.30 - 0.30 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TNFAIP3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TNFAIP3 antibody images, references, products related to ab45366 and other tools.
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TNFAIP3 antibody images:
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 Western blot - TNFAIP3 antibody (ab45366)
TNFAIP3 antibody (ab45366) at 1 µg/ml + Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab3951) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 89 kDa
Observed band size : 89 kDa
Additional bands at : 50 kDa,75 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to TNFAIP3
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TNFAIP3 antibody - more information
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Customer reviews (feedback) regarding TNFAIP3 antibody |
Customer FAQs regarding TNFAIP3 antibody |
Protocols for TNFAIP3 antibody |
Price and availability of products related to TNFAIP3 antibody |
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TNFAIP3 antibody - related products:
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TNFAIP3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about TNFAIP3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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