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I have used ab74037 to detect TNFAIP3 from human stem cells. I have used this antibody a few years ago in the same experiement (western blotting) with similar samples and it worked as expected. However this recent batch gives no bands. I used primary at 1:250, 1:500 and 1:1000 with no differences. Other antibodies have been used successfully with the same samples |
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ANSWER: |
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab45366. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research. |
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I have used ab74037 to detect TNFAIP3 from human stem cells. I have used this antibody a few years ago in the same experiement (western blotting) with similar samples and it worked as expected. However this recent batch gives no bands. I used primary at 1:250, 1:500 and 1:1000 with no differences. Other antibodies have been used successfully with the same samples. |
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ANSWER: |
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab92324. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunofluorescence analysis of TNFAIP3 in HepG2 cells using ab74037 at a 1/500 dilution.
Left image: untreated.
Right image: treated with immunizing peptide.
All lanes : Anti-TNFAIP3 antibody (ab74037) at 1/500 dilution
Lane 1 : Extracts from RAW264.7 cells
Lane 2 : Extracts from RAW264.7 cells with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 90 kDa
Observed band size : 90 kDa
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
ab74037, at 1/50, staining TNFAIP3 in mouse tissue sections by immunohistochemistry (frozen sections).
This image is courtesy of an anonymous Abreview.
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