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Products:Immunology >> Innate Immunity >> Cytokines >> TNF Superfamily
Overview
Product name
Anti-TNFAIP3 antibody
See all TNFAIP3 products (7) ...
Description
Rabbit polyclonal to TNFAIP3
Specificity
This antibody detects endogenous levels of total TNFAIP3 protein.
Tested applications
IHC-Fr, ELISA, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A synthesized peptide derived from the internal region of human TNFAIP3.
Positive control
HepG2 cells and extracts from RAW264.7 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: 1/20000.
ICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000. Predicted molecular weight: 90 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
Sequence similarities
Belongs to the peptidase C64 family.
Contains 7 A20-type zinc fingers.
Contains 1 OTU domain.
Domain
The A20-type zinc fingers mediate the ubiquitin ligase activity.
The OTU domain mediates the deubiquitinase activity.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionP21580
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A20 antibody
- MGC104522 antibody
- MGC138687 antibody
see all
Database links
- Entrez Gene: 7128 Human
- Entrez Gene: 21929 Mouse
- Omim: 191163 Human
- SwissProt: P21580 Human
- SwissProt: Q60769 Mouse
- Unigene: 211600 Human
- Unigene: 116683 Mouse
Anti-TNFAIP3 antibody images:
Immunocytochemistry/ Immunofluorescence - TNFAIP3 antibody (ab74037)
Immunofluorescence analysis of TNFAIP3 in HepG2 cells using ab74037 at a 1/500 dilution.
Left image: untreated.
Right image: treated with immunizing peptide.
Western blot - TNFAIP3 antibody (ab74037)
All lanes : Anti-TNFAIP3 antibody (ab74037) at 1/500 dilution
Lane 1 : Extracts from RAW264.7 cells
Lane 2 : Extracts from RAW264.7 cells with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 90 kDa
Observed band size : 90 kDa
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Frozen sections) - TNFAIP3 antibody (ab74037)
ab74037, at 1/50, staining TNFAIP3 in mouse tissue sections by immunohistochemistry (frozen sections).
This image is courtesy of an anonymous Abreview.
References for Anti-TNFAIP3 antibody (ab74037)
ab74037 has not yet been referenced specifically in any publications.
Publishing research using ab74037? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"