Anti-TNFRSF14/HVEM antibody (ab62462)
Key features and details
- Rabbit polyclonal to TNFRSF14/HVEM
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TNFRSF14/HVEM antibody
See all TNFRSF14/HVEM primary antibodies -
Description
Rabbit polyclonal to TNFRSF14/HVEM -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TNFRSF14/HVEM (C terminal).
-
Positive control
- Raji cell lysate. Raji cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 31 kDa).
|
|
ICC |
Use a concentration of 10 - 20 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 31 kDa). |
ICC
Use a concentration of 10 - 20 µg/ml. |
Target
-
Function
Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. -
Tissue specificity
Widely expressed, with the highest expression in lung, spleen and thymus. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 8764 Human
- Omim: 602746 Human
- SwissProt: Q92956 Human
- Unigene: 512898 Human
-
Alternative names
- HVEML antibody
- ATAR antibody
- CD270 antibody
see all
Images
-
All lanes : Anti-TNFRSF14/HVEM antibody (ab62462) at 2 µg/ml
Lane 1 : Raji cell lysate with no blocking peptide
Lane 2 : Raji cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 31 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted? -
Immunocytochemistry of TNFRSF14/HVEM in Raji cells with ab62462 antibody at 10 µg/ml.
-
Immunofluorescence of TNFRSF14/HVEM in Raji cells using ab62462 at 20 ug/ml.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab62462 has been referenced in 1 publication.
- Duan L et al. HVEM/HIF-1a promoted proliferation and inhibited apoptosis of ovarian cancer cells under hypoxic microenvironment conditions. J Ovarian Res 13:40 (2020). PubMed: 32312328