Recombinant Anti-TPR antibody [EPR8982] (ab170940)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8982] to TPR
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TPR antibody [EPR8982]
See all TPR primary antibodies -
Description
Rabbit monoclonal [EPR8982] to TPR -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human TPR (Cysteine residue). The exact sequence is proprietary.
Database link: P12270 -
Positive control
- HeLa + Camptothecin, HeLa, HeLa + Staurosporine, HeLa, 293T and Jurkat whole cell lysate (ab7899), HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8982 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/250.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 267 kDa.
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Notes |
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ICC/IF
1/100 - 1/250. |
WB
1/1000 - 1/5000. Predicted molecular weight: 267 kDa. |
Target
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Function
Component of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint. -
Tissue specificity
Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney. -
Involvement in disease
Defects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
Note=Involved in tumorigenic rearrangements with the MET or RAF genes. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase. - Information by UniProt
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Database links
- Entrez Gene: 7175 Human
- Omim: 189940 Human
- SwissProt: P12270 Human
- Unigene: 279640 Human
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Alternative names
- Nucleoprotein TPR antibody
- tpr antibody
- TPR_HUMAN antibody
- translocated promoter region (to activated MET oncogene) antibody
Images
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All lanes : Anti-TPR antibody [EPR8982] (ab170940) at 1/1000 dilution
Lane 1 : HeLa + Camptothecin cell lysates
Lanes 2 & 4 : HeLa cell lysates
Lane 3 : HeLa + Staurosporine cell lysates
Lane 5 : 293T cell lysates
Lane 6 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 267 kDa -
Immunofluorescence analysis of HeLa cells labeling TPR with ab170940 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab170940 has been referenced in 3 publications.
- Li K et al. Stabilization of oncogenic transcripts by the IGF2BP3/ELAVL1 complex promotes tumorigenicity in colorectal cancer. Am J Cancer Res 10:2480-2494 (2020). PubMed: 32905413
- Wan J et al. Inhibitory effect of Ubenimex combined with fluorouracil on multiple drug resistance and P-glycoprotein expression level in non-small lung cancer. J Cell Mol Med 24:12840-12847 (2020). PubMed: 32945069
- Myers KN et al. The bornavirus-derived human protein EBLN1 promotes efficient cell cycle transit, microtubule organisation and genome stability. Sci Rep 6:35548 (2016). WB ; Human . PubMed: 27739501