Overview
Product nameTPR protein (Tagged-His Tag)
Protein descriptionRecombinant fragment, corresponding to amino acids 69-668 (600 amino acids) of Human TPR with N terminal His tag. MWt: 69 kDa; UniProt ID = P12270
Expression hostE. coli
Properties
Purification notesPurity level >85%
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography.
In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag.
In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight.
This may be as a result of unequally distributed charges in the amino acid sequence.
Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
FormLyophilised:Reconstitute with 103 µl aqua dest.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Concentration information loading...
Sequence notesVNETRECQSLRLELEKLNNQLKALTEKNKELEIAQDRNIA IQSQFTRTKEELEAEK
RDLIRTNERLSQELEYLTEDVK RLNEKLKESNTTKGELQLKLDELQASDVSVKYRE
KRLE QEKELLHSQNTWLNTELKTKTDELLALGREKGNEILELKC NLENKKEEVSRL
EEQMNGLKTSNEHLQKHVEDLLTKLK EAKEQQASMEEKFHNELNAHIKLSNLYKSA
ADDSEAKS NELTRAVEELHKLLKEAGEANKAIQDHLLEVEQSKDQMEK EMLEKIGR
LEKELENANDLLSATKRKGAILSEEELAAM SPTAAAVAKIVKPGMKLTELYNAYVE
TQDQLLLEKLEN KRINKYLDEIVKEVEAKAPILKRQREEYERAQKAVASLSV KLEQ
AMKEIQRLQEDTDKANKQSSVLERDNRRMEIQVK DLSQQIRVLLMELEEARGNHVI
RDEEVSSADISSSSEV ISQHLVSYRNIEELQQQNQRLLVALRELGETREREEQETT SSKITELQLKLESALTELEQLRKSRQHQMQLVDSIVRQ RDMYRILLSQTTGVAIPL
HASSLDDVSLASTPKRPSTS QTVSTPAPVPVIESTEAIEA
(Amino acid sequence (Sequence determined by 5' Sequencing))
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab91741
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
|
Application notesSDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
-
Alternative names
Nucleoprotein TPRtprTPR_HUMANtranslocated promoter region (to activated MET oncogene)
FunctionComponent of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint.
Tissue specificityHighest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
Involvement in diseaseDefects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
Note=Involved in tumorigenic rearrangements with the MET or RAF genes.
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase.
TPR protein (Tagged-His Tag) images
References for TPR protein (Tagged-His Tag) (ab91741)
ab91741
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - TPR protein (Tagged-His Tag) (ab91741)The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.