Products:Stem Cells >> Embryonic Stem Cells >> Surface Molecules
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ab16288 has been referenced in 18 publications.
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All lanes : Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288) at 10 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (HRP) (ab5930) at 1/5000 dilution
Predicted band size : 235 kDa
Observed band size : 250 kDa (why is the actual band size different from the predicted?)
As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody.
Anti-TRA-1-60 antibody, ab16288, can be used as a marker of human embryonic stem cells. DAPI staining is shown in the top panel while TRA-1-81 staining with ab16288 is shown in the lower panel.
This image is courtesy of an Abreview submitted by Dr Maria Keramari
ab16288 staining the Embryonic Stem Cell Marker in HUES7 cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked in 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody, dilution 1/500 (1% Serum, 0.1% Triton in PBS), for 1 hour at 37°C. An Alexa Fluor® 647-conjugated Goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.
This image is courtesy of an Abreview submitted by Ms Fiona Lewis
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