TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288)

Product Name 

TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker

Product type 

Primary antibodies

Description 

Mouse monoclonal [TRA-1-60] to TRA-1-60 (R) - Embryonic Stem Cell Marker

Immunogen 

Human embryonal carcinoma cell line 2102Ep cl.2A6.

Reacts with 
(species key)

Hu

Specificity 

This antibody reacts with the TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a neuraminidase resistant epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule. The epitopes for both TRA-1-81 and TRA-1-60 are not confined to human EC cells, but are also found on several neoplastic and normal tissues, though in a different pattern for each antibody. TRA-1-81 immunoprecipitates a 420kDa and a 250kDa protein and TRA-1-60 immunoprecipitates a 235kDa and a 410kDa protein from lysates of human EC cells.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-Fr, IP, RIA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at a concentration of 10 - 20 µg/ml.
ICC: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane

Research areas 

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Stem Cells >> Embryonic Stem Cells >> Surface Molecules

Relevance 

The monoclonal antibody TRA-1-60 is a marker of Human Embryonic Stem, Germ and Carcinoma Cells but Not Mouse Embryonic Stem, Germ or Carcinoma Cells. The core protein recognised by TRA-1-60 has not been identified but the antibody is thought to recognise carbohydrate epitopes associated with a high molecular weight proteoglycan. The TRA antibodies are named after the Battle of Trafalgar; TRA does not stand for "Tumor Rejection Antigen" as is believed by some.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

TRA-1-60

Myeloma 

P3x66Ag.8-Sp2/0

Isotype 

IgM

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS

Form 

Liquid

Concentration 

2.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

The myeloma was fused with a spleen from a Balb/c mouse.

See below for TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker images, references, products related to ab16288 and other tools.

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References for TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288)

  This product has been used in: (two most recent references)

Mizrak SC et al. Embryonic stem cell-like cells derived from adult human testis. Hum Reprod 25:158-67 (2010). ICC/IF; Human. PubMed: 19815622

Lock LT & Tzanakakis ES Expansion and differentiation of human embryonic stem cells to endoderm progeny in a microcarrier stirred-suspension culture. Tissue Eng Part A 15:2051-63 (2009). PubMed: 19196140

See all 9 publication references for this product.


If you publish research using ab16288 please let us know so that we can cite the reference on this datasheet.

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