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Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288)

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Overview

Product name

Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker

Description

Mouse monoclonal [TRA-1-60] to TRA-1-60 (R) - Embryonic Stem Cell Marker

Specificity

This antibody reacts with the TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a neuraminidase resistant epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule. The epitopes for both TRA-1-81 and TRA-1-60 are not confined to human EC cells, but are also found on several neoplastic and normal tissues, though in a different pattern for each antibody. TRA-1-81 immunoprecipitates a 420kDa and a 250kDa protein and TRA-1-60 immunoprecipitates a 235kDa and a 410kDa protein from lysates of human EC cells.

Tested applications

ICC/IF, Flow Cyt, WB, IP, ICC, RIA, IHC-Frmore details

Cross reactivity

Reacts with

Rabbit, Human

Immunogen

Human embryonal carcinoma cell line 2102Ep cl.2A6.

General notes

The myeloma was fused with a spleen from a Balb/c mouse.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Purity

Tissue culture supernatant

Purification notes

Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS

Clonality

Monoclonal

Clone number

TRA-1-60

Myeloma

P3x66Ag.8-Sp2/0

Isotype

IgM

Research areas

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Stem Cells >> Embryonic Stem Cells >> Surface Molecules

  • Western blot - TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288)Western blot - TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - TRA-1-60 antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288)Immunocytochemistry/ Immunofluorescence - TRA-1-60 antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288)Immunocytochemistry/ Immunofluorescence - TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab16288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The monoclonal antibody TRA-1-60 is a marker of Human Embryonic Stem, Germ and Carcinoma Cells but Not Mouse Embryonic Stem, Germ or Carcinoma Cells. The core protein recognised by TRA-1-60 has not been identified but the antibody is thought to recognise carbohydrate epitopes associated with a high molecular weight proteoglycan. The TRA antibodies are named after the Battle of Trafalgar; TRA does not stand for "Tumor Rejection Antigen" as is believed by some.

Cellular localization

Cell Membrane

Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker images:

  Western blot - TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288)

Western blot - TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288)

All lanes : Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288) at 10 µg/ml

Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (HRP) (ab5930) at 1/5000 dilution

Predicted band size : 235 kDa
Observed band size : 250 kDa (why is the actual band size different from the predicted?)


As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody.

  Immunocytochemistry/ Immunofluorescence - TRA-1-60 antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288)

Immunocytochemistry/ Immunofluorescence - TRA-1-60 antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288)

Anti-TRA-1-60 antibody, ab16288, can be used as a marker of human embryonic stem cells. DAPI staining is shown in the top panel while TRA-1-81 staining with ab16288 is shown in the lower panel.

This image is courtesy of an Abreview submitted by Dr Maria Keramari

See Abreview

  Immunocytochemistry/ Immunofluorescence - TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288)

Immunocytochemistry/ Immunofluorescence - TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288)

ab16288 staining  the Embryonic Stem Cell Marker in HUES7 cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked in 10% serum  for 1 hour at 24°C.  Samples were incubated with primary antibody, dilution 1/500 (1% Serum, 0.1% Triton in PBS), for 1 hour at 37°C. An Alexa Fluor® 647-conjugated Goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.

This image is courtesy of an Abreview submitted by Ms Fiona Lewis

See Abreview

References for Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker (ab16288)

This product has been referenced in:

  • Anchan RMet al. Amniocytes can serve a dual function as a source of iPS cells and feeder layers. Hum Mol Genet 20:962-74 (2011). ICC/IF; Human.Read more (PubMed: 21156717) »
  • Bone HKet al. A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3. J Cell Sci 124:1992-2000 (2011). Flow Cyt; Human.Read more (PubMed: 21610099) »

See all 17 publications for this product

Publishing research using ab16288? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"