Products:Stem Cells >> Embryonic Stem Cells >> Surface Molecules
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ab16289 has been referenced in 14 publications.
Publishing research using ab16289? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289) at 3 µg/ml + MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µg
Secondary
Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (HRP) (ab5930) at 1/5000 dilution
developed using the ECL technique
Predicted band size : 200-400 kDa
Exposure time : 3 minutes
ab16289 at 1/100 staining human embryonic stem cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% goat serum prior to incubation with the antibody for 1 hour. An Alexa-Fluor 488 conjugated Goat anti-mouse IgM was used as the secondary antibody.
DAPI staining is shown in the top panel while TRA-1-81 staining with ab16289 is shown in the lower panel.
This image is courtesy of an Abreview submitted by Dr Maria Keramari
ab16289 staining TRA-1-81 from human embryonic stem cells by immunocytochemistry/immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in Triton prior to blocking in 10% serum for 1 hour at 24ºC. The primary antibody was diluted 1/500 and incubated with the sample for 1 hour at 37ºC. An Alexafluor 647 goat polyclonal to mouse IgM, diluted 1/200, was used as the secondary.
This image is courtesy of an Abreview submitted by Fiona Lewis
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