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Products:Stem Cells >> Embryonic Stem Cells >> Surface Molecules
Anti-TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab16289 for help.
There are no answered questions for ab16289
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)](/ps/datasheet/Images/16/ab16289/ab16289 3 mins copy.jpg)
Western blot - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)
Anti-TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289) at 3 µg/ml + MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µg
Secondary
Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (HRP) (ab5930) at 1/5000 dilution
developed using the ECL technique
Predicted band size : 200-400 kDa
Exposure time : 3 minutes
![Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)](/ps/datasheet/Images/16/ab16289/ab16289_1.jpg)
Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)
ab16289 at 1/100 staining human embryonic stem cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% goat serum prior to incubation with the antibody for 1 hour. An Alexa-Fluor 488 conjugated Goat anti-mouse IgM was used as the secondary antibody.
DAPI staining is shown in the top panel while TRA-1-81 staining with ab16289 is shown in the lower panel.
This image is courtesy of an Abreview submitted by Dr Maria Keramari
![Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)](/ps/datasheet/images/16/ab16289/TRA-1-81-Primary-antibodies-ab16289-2.jpg)
Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)
ab16289 staining TRA-1-81 from human embryonic stem cells by immunocytochemistry/immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in Triton prior to blocking in 10% serum for 1 hour at 24ºC. The primary antibody was diluted 1/500 and incubated with the sample for 1 hour at 37ºC. An Alexafluor 647 goat polyclonal to mouse IgM, diluted 1/200, was used as the secondary.
This image is courtesy of an Abreview submitted by Fiona Lewis
![TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker for ICC/IF in Human (16289)](/ps/datasheet/images/16/ab16289/TRA-1-81-Primary-antibodies-ab16289-1.jpg)
TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker for ICC/IF in Human (16289)
![TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker for ICC/IF in Human (16289)](/ps/Reviews/Images/ab6345_23423.jpg)
TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker for ICC/IF in Human (16289)
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