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Anti-TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)

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Overview

Product name

Anti-TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker
See all TRA-1-81 products (2) ...

Description

Mouse monoclonal [TRA-1-81] to TRA-1-81 - Embryonic Stem Cell Marker

Specificity

This antibody reacts with TRA-1-81 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells.Both the TRA-1-81 and TRA-1-60 monoclonal antibodies recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule. The epitopes for both TRA-1-81 and TRA-1-60 are not confined to human EC cells, but are also found on several neoplastic and normal tissues, though in a different pattern for each antibody. TRA-1-81 immunoprecipitates a 420kD and a 250kD protein and TRA-1-60 immunoprecipitate a 235kD and a 410kD protein from lysates of human EC cells.

Tested applications

ICC/IF, Flow Cyt, WB, IP, ICC, RIA, IHC-Frmore details

Cross reactivity

Reacts with

Rabbit, Human

Does not react with

Mouse

Immunogen

Human embryonal carcinoma cell line 2102Ep cl.2A6.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.5

Purity

Tissue culture supernatant

Purification notes

Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS

Clonality

Monoclonal

Clone number

TRA-1-81

Myeloma

P3x66Ag.8-Sp2/0

Isotype

IgM

Research areas

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Stem Cells >> Embryonic Stem Cells >> Surface Molecules

  • Western blot - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)Western blot - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab16289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The Podocalyxin gene encodes a member of the sialomucin protein family, and the encoded protein was originally identified as an important component of glomerular podocytes. Other biological activities of the encoded protein include: playing a role in hematopoetic cell differentiation, binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements and being expressed in vascular endothelium cells and binding to L-selectin.

Cellular localization

Membrane; Single-pass type I membrane protein

Alternative names

  • Gp200 antibody
  • PC antibody
  • PCLP antibody
  • PCLP1 antibody

Database links

Anti-TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker images:

  Western blot - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)

Western blot - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)

Anti-TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289) at 3 µg/ml + MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µg

Secondary
Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain (HRP) (ab5930) at 1/5000 dilution
developed using the ECL technique

Predicted band size : 200-400 kDa


Exposure time : 3 minutes

  Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)

Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)

ab16289 at 1/100 staining human embryonic stem cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% goat serum prior to incubation with the antibody for 1 hour. An Alexa-Fluor 488 conjugated Goat anti-mouse IgM was used as the secondary antibody.

DAPI staining is shown in the top panel while TRA-1-81 staining with ab16289 is shown in the lower panel.

This image is courtesy of an Abreview submitted by Dr Maria Keramari

See Abreview

  Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)

Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)

ab16289 staining TRA-1-81 from human embryonic stem cells by immunocytochemistry/immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in Triton prior to blocking in 10% serum for 1 hour at 24°C. The primary antibody was diluted 1/500 and incubated with the sample for 1 hour at 37°C. An Alexafluor 647 goat polyclonal to mouse IgM, diluted 1/200, was used as the secondary.

This image is courtesy of an Abreview submitted by Fiona Lewis

See Abreview

References for Anti-TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)

This product has been referenced in:

  • Huang Bet al. A Virus-Free Poly-Promoter Vector Induces Pluripotency in Quiescent Bovine Cells under Chemically Defined Conditions of Dual Kinase Inhibition. PLoS One 6:e24501 (2011). ICC/IF; Cow.Read more (PubMed: 21912700) »
  • Okahara-Narita Jet al. Induction of pluripotent stem cells from fetal and adult cynomolgus monkey fibroblasts using four human transcription factors. Primates : (2011). ICC/IF; Cynomolgus Monkey.Read more (PubMed: 22075965) »

See all 14 publications for this product

Publishing research using ab16289? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"