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Western blot - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)
![Western blot - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)](/ps/datasheet/images/ab16289 3 mins copy.jpg)
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Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)
![Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)](/ps/datasheet/images/ab16289_1.jpg)
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Product Name
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TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [TRA-1-81] to TRA-1-81 - Embryonic Stem Cell Marker
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Immunogen
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Human embryonal carcinoma cell line 2102Ep cl.2A6.
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Reacts with
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Hu
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Does not react with
(species key)
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Ms
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Specificity
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This antibody reacts with TRA-1-81 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells.Both the TRA-1-81 and TRA-1-60 monoclonal antibodies recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule. The epitopes for both TRA-1-81 and TRA-1-60 are not confined to human EC cells, but are also found on several neoplastic and normal tissues, though in a different pattern for each antibody. TRA-1-81 immunoprecipitates a 420kD and a 250kD protein and TRA-1-60 immunoprecipitate a 235kD and a 410kD protein from lysates of human EC cells.
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IP, RIA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions Flow Cyt: Use at a concentration of 10 - 20 µg/ml. ICC: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. RIA: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 200-400 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular Stem Cells >> Embryonic Stem Cells >> Surface Molecules
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Relevance
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The monoclonal antibody TRA-1-81 is a marker of Human Embryonic Stem, Germ and Carcinoma Cells but Not Mouse Embryonic Stem, Germ or Carcinoma Cells. The core protein recognised by TRA-1-81 has not been identified but the antibody is thought to recognise carbohydrate epitopes associated with a high molecular weight proteoglycan. The TRA antibodies are named after the Battle of Trafalgar; TRA does not stand for "Tumor Rejection Antigen" as is believed by some.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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TRA-1-81
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Myeloma
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P3x66Ag.8-Sp2/0
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Isotype
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IgM
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: PBS, pH 7.5 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker images, references, products related to ab16289 and other tools.
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TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker images:
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Western blot - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)
TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289) at 3 µg/ml + MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µg
Secondary Goat polyclonal to Mouse IgM - mu chain specific - F(ab)2 Fragment (HRP) (ab5930) at 1/5000 dilution developed using the ECL technique
Predicted band size : 200-400 kDa
Exposure time : 3 minutes
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Immunocytochemistry/ Immunofluorescence - TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)
ab16289 at 1/100 staining human embryonic stem cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% goat serum prior to incubation with the antibody for 1 hour. An Alexa-Fluor 488 conjugated Goat anti-mouse IgM was used as the secondary antibody. DAPI staining is shown in the top panel while TRA-1-81 staining with ab16289 is shown in the lower panel. This image is courtesy of an Abreview submitted by Dr Maria Keramari
See Abreview
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References for TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker (ab16289)
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This product has been used in: (two most recent references)
Lu M et al. Enhanced generation of hematopoietic cells from human hepatocarcinoma cell-stimulated human embryonic and induced pluripotent stem cells. Exp Hematol 37:924-36 (2009). PubMed: 19481135
Lock LT & Tzanakakis ES Expansion and differentiation of human embryonic stem cells to endoderm progeny in a microcarrier stirred-suspension culture. Tissue Eng Part A 15:2051-63 (2009). PubMed: 19196140
See all 7 publication references for this product.
If you publish research using ab16289 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to TRA-1-81
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TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker - more information
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TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker - related products:
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TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker - other tools:
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Contact Abcam with a Technical Enquiry about TRA-1-81 antibody [TRA-1-81] - Embryonic Stem Cell Marker |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"