TRAIL R2 Human ELISA Kit (ab113340)
- Product nameTRAIL R2 Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 2 pg/ml
- Range2.46 pg/ml - 600 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.22 88% - 107% Serum 94.88 83% - 102% Plasma 78.79 68% - 97%
- Species reactivityReacts with: Human
- Product overview
ab113340 Human TRAIL R2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TRAIL R2 in serum, plasma, cell culture supernatant. This assay employs an antibody specific for human TRAIL R2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TRAIL R2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TRAIL R2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TRAIL R2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A Item D 1 x 30ml Assay Diluent B Item E 1 x 15ml Detection Antibody TRAIL R2 Item F 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml TRAIL R2 Microplate (12 strips x 8 wells) coated with anti-Human TRAIL R2 Item A 1 unit TRAIL R2 Standard Human (recombinant) 2 vials
- SwissProt: O14763 Human
Our Abpromise guarantee covers the use of ab113340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for TRAIL R2 Human ELISA Kit (ab113340)
ab113340 has not yet been referenced specifically in any publications.