![Flow Cytometry - Anti-TRAIL antibody [B-S23] (Phycoerythrin) (ab47230) image](#TRAIL-Primary-antibodies-ab47230-12.jpg)
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Product name
Anti-TRAIL antibody [B-S23] (Phycoerythrin)
See all TRAIL products (23) ...
Description
Mouse monoclonal [B-S23] to TRAIL (Phycoerythrin)
Conjugation
Phycoerythrin
Specificity
ab47230 recognises the TRAIL, APO2L antigen.
Tested applications
Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant human TRAIL
Properties
Form
Lyophilised:
Reconstitute with 1 ml deionised water.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 5% BSA, PBS
Concentration
Concentration information loading...
Purity
Ion Exchange Chromatography
Clonality
Monoclonal
Clone number
B-S23
Myeloma
x63-Ag8.653
Isotype
IgG1
Research areas
Cardiovascular >> Atherosclerosis >> Apoptosis
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> TRAIL
Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
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Flow Cytometry - Anti-TRAIL antibody [B-S23] (Phycoerythrin) (ab47230)
![Flow Cytometry - Anti-TRAIL antibody [B-S23] (Phycoerythrin) (ab47230) image](/ps/datasheet/images/47/ab47230/TRAIL-Primary-antibodies-ab47230-12.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab47230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 10µl for 106 cells. or 100 µl of whole blood.
Target
Function
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Tissue specificity
Widespread; most predominant in spleen, lung and prostate.
Sequence similarities
Belongs to the tumor necrosis factor family.
Cellular localization
Membrane.
Target information above from: UniProt accessionP50591
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apo 2 ligand antibody
- Apo 2L antibody
- Apo-2 ligand antibody
see all
Database links
- Entrez Gene: 8743 Human
- Omim: 603598 Human
- SwissProt: P50591 Human
- Unigene: 478275 Human
Anti-TRAIL antibody [B-S23] (Phycoerythrin) images:
Flow Cytometry - Anti-TRAIL antibody [B-S23] (Phycoerythrin) (ab47230)
ab47230 staining TRAIL in human peripheral blood monocytes by Flow Cytometry.Cells were fixed in paraformaldehyde, permeabilized using 0.1% saponin/ PBS.ab47230 used at a 1/10 dilution.1 = non stimulated2 = INF gamma stimulated 100U/ml3 = LPS stimulated 100ng/ml
Image courtesy of an anonymous Abreview.
References for Anti-TRAIL antibody [B-S23] (Phycoerythrin) (ab47230)
This product has been referenced in:
- Rana K et al. Delivery of apoptotic signal to rolling cancer cells: a novel biomimetic technique using immobilized TRAIL and E-selectin. Biotechnol Bioeng 102:1692-702 (2009). Human.Read more (PubMed: 19073014) »
See 1 publication for this product
Publishing research using ab47230? Please let us know so that we can cite the reference in this datasheet
![Anti-TRAIL antibody [B-S23] (Phycoerythrin) for Flow Cytometry in Human (47230)](/ps/datasheet/images/47/ab47230/TRAIL-Primary-antibodies-ab47230-11.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"