Anti-TRAK1 antibody (ab28751)
Key features and details
- Goat polyclonal to TRAK1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRAK1 antibody -
Description
Goat polyclonal to TRAK1 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Synthetic peptide:
GAKLSKQTSLR,
corresponding to C terminal amino acids 943-953 of Human TRAK1. -
Positive control
- Human Heart Lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
Use a concentration of 0.3 - 1.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 106 kDa).
A 1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.3 - 1.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 106 kDa). A 1 hour primary incubation is recommended for this product. |
Target
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Relevance
The specific function of TRAK1 remains unknown. It interacts with O-GlcNAc transferase, RHOT1/Miro-1 and RHOT2/Miro-2. It shows high expression in spinal cord and moderate expression in all other tissues and specific brain regions examined. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 22906 Human
- Omim: 608112 Human
- SwissProt: Q9UPV9 Human
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Alternative names
- 106 kDa O GlcNAc transferase interacting protein antibody
- KIAA1042 antibody
- OGT(O Glc NAc transferase) interacting protein 106 KDa antibody
see all
Images
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Anti-TRAK1 antibody (ab28751) at 0.3 µg/ml + human heart lysate (RIPA buffer, 30µg total protein per lane)
Predicted band size: 106 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Primary incubation was for 1 hour.
Detected by western blot using chemiluminescence.
Datasheets and documents
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Datasheet download
References (2)
ab28751 has been referenced in 2 publications.
- Feng Y et al. Dl-3-n-Butylphthalide Alleviates Demyelination and Improves Cognitive Function by Promoting Mitochondrial Dynamics in White Matter Lesions. Front Aging Neurosci 13:632374 (2021). PubMed: 33762923
- Ogawa F et al. DISC1 complexes with TRAK1 and Miro1 to modulate anterograde axonal mitochondrial trafficking. Hum Mol Genet N/A:N/A (2013). WB, ICC/IF ; Human . PubMed: 24092329