Anti-TRANK1 antibody (ab122283)
Key features and details
- Rabbit polyclonal to TRANK1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRANK1 antibody -
Description
Rabbit polyclonal to TRANK1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: CKLADSIKAI CNAYNRGLSC VLRKKLKGIN KGQVSANMKI QKRIPRCYVE DTEAEKGREH VNPEYFPPAS AVETEYNIMK FHSFST, corresponding to amino acids 905-990 of Human TRANK1 (UniProt ID: O15050).
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Positive control
- IHC: Human fallopian tube and duodenum tissue. ICC: Human cell line U-2 OS
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Contains 6 ANK repeats.
Contains 4 TPR repeats. - Information by UniProt
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Database links
- Entrez Gene: 9881 Human
- SwissProt: O15050 Human
- Unigene: 170999 Human
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Alternative names
- KIAA0342 antibody
- LBA1 antibody
- Lupus brain antigen 1 homolog antibody
see all
Images
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ab122283, at a 1/200 dilution, staining TRANK1 in paraffin embedded Human duodenum tissue by Immunohistochemistry.
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ab122283, at a 1/200 dilution, staining TRANK1 in paraffin embedded Human fallopian tube by Immunohistochemistry.
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122283 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122283 has not yet been referenced specifically in any publications.