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Products:Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Overview
Product name
Anti-TRAP220/MED1 antibody
See all TRAP220/MED1 products (8) ...
Description
Mouse polyclonal to TRAP220/MED1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Fusion protein: DCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDP ADLIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGF GEEYFDESSQSGDNDDFKGF, corresponding to amino acids 799/898 of Human PPAR binding protein
DCPAIGTPLR DSSSSGHSQS TLFDSDVFQT NNNENPYTDP ADLIADAAGS PSSDSPTNHF FHDGVDFNPD LLNSQSQSGF GEEYFDESSQ SGDNDDFKGF
General notes
Produced from outbred CD1 mice
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> RNA polymerase
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
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Western blot - PPAR binding protein antibody (ab22229)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 168 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Target
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the Mediator complex subunit 1 family.
Post-translational
modifications
Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
Target information above from: UniProt accessionQ15648
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activator-recruited cofactor 205 kDa component antibody
- ARC205 antibody
- CRSP1 antibody
see all
Database links
- Entrez Gene: 5469 Human
- Omim: 604311 Human
- SwissProt: Q15648 Human
- SwissProt: Q6P9H7 Human
- Unigene: 643754 Human
Anti-TRAP220/MED1 antibody images:
Western blot - PPAR binding protein antibody (ab22229)
All lanes : Anti-TRAP220/MED1 antibody (ab22229) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Predicted band size : 168 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
References for Anti-TRAP220/MED1 antibody (ab22229)
ab22229 has not yet been referenced specifically in any publications.
Publishing research using ab22229? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"